Recombinant DNA Flashcards
1
Q
What is Recombinant DNA Technology?
A
Recombinant DNA technology involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest
2
Q
What is Recombinant DNA Technology?
A
Recombinant DNA technology involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest
3
Q
what type of species can be used in recombinant DNA ?
A
recombinant DNA molecules can originate from any species
3
Q
what type of species can be used in recombinant DNA ?
A
recombinant DNA molecules can originate from any species
4
Q
how do you produce rDNA ?
A
- The desired site of clevage on the host plasmid is identified
- This clevage is spliced by restriction endonucleases
- Sticky ends are then put through the stage of annealing to join it to another DNA
4
Q
how do you produce rDNA ?
A
- The desired site of clevage on the host plasmid is identified
- This clevage is spliced by restriction endonucleases
- Sticky ends are then put through the stage of annealing to join it to another DNA
5
Q
Recombinant DNA - Applications
A
- Recombinant Human Insulin
- Recombinant Human Growth Hormone
- Recombinant Blood Clotting Factor VIII
- Golden Rice
- Herbicide-resistant Crops
