Transcription: RNA Synthesis

Question 1

Define: Transcription

Answer 1

Formation of mRNA from a DNA template

Question 2

5 differences of Transcription from DNA Replication

Answer 2

only 1 template strand, RNA Polymerase, no RNA Primers needed, result is a SS-mRNA, only small units are transcribed

Question 3

2 types of mRNA

Answer 3

Monocistronic & Polycistronic

Question 4

3 steps in Transcription

Answer 4

Initiation , Elongation, Termination

Question 5

During initiation, this is the site where the RNA Polymerase holoenzyme attaches

Answer 5

promoter region

Question 6

2 types of promoter regions in bacteria

Answer 6

TTGACA (-35 bp) & Pribnow box (TATAAT) (-10 bp)

Question 7

T/F: The leader sequence is translated

Answer 7

False, The leader sequence is not translated

Question 8

position of leader sequence

Answer 8

(-) 25-150 bp at the 5’ end of the initiation codon

Question 9

position of trailer sequence

Answer 9

at the 3’ end; follows the termination codon

Question 10

Function of RNA Polymerase

Answer 10

unwinds the DS-DNA to form a transcription bubble

Question 11

Components of holoenzyme

Answer 11

alpha, beta, beta prime, omega. sigma

Question 12

term to define the holoenzyme when the sigma already dissociated from it

Answer 12

RNA Polymerase core enzyme

Question 13

direction of elongation

Answer 13

5’-3’ direction

Question 14

major enzyme involved during Elongation

Answer 14

RNA Polymerase core enzyme

Question 15

define: Transcript

Answer 15

code that signals the separation and release of RNA strand

Question 16

T/F: Sigma factor is needed during the Elongation

Answer 16

False, only the core enzyme is needed

Question 17

2 types of Termination

Answer 17

Intrinsic termination & Rho-dependent Termination

Question 18

Signal to initiate transcription

Answer 18

Detachment of Sigma subunit from RNA Polymerase holoenzyme