Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MD2S3: Molecular Biology > Transcription in Prokaryotes > Flashcards

Transcription in Prokaryotes Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

What are the products of transcription?

A

mRNA
rRNA
tRNA
ncRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the requirements for RNA synthesis?

A

DNA Template
4 NTPs
Mg2+ or Mn2+
RNA Polymerase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the enzymes capable of RNA synthesis?

A

RNA Polymerase (Transcription)
Primase (for DNA replication)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

RNA is synthesized from its ____-end to its ____-end

A

__5’__
__3’__

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

RNA is complementary to the ____ strand and identical to the ____ strand (with U replacing T)

A

__antisense/minus/DNA template__
__sense/plus/coding__

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does transcription by RNA polymerase involve?

A

A Core Enzyme
Several Auxiliary Proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the 5 peptides which forms the core enzyme?

A

2α
1β
1β’
1ω

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the functions of the five peptides forming the core enzyme?

A

Enzyme Assembly
Template Binding
5’ → 3’ Polymerase Activity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What can the core enzyme not accomplish?

A

It cannot recognize the promotor region of the DNA template

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is a Holoenzyme?

A

RNA polymerase + Sigma Factor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Function of Holoenzyme

A

Capable of recognizing promotor region on DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are the steps of RNA Synthesis?

A

Initiation
Elongation
Termination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Initiation
What happens during initiation?

A

RNA Polymerase Holoenzyme binds to Promotor Region

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Initiation
What are the Prokaryotic Promotors?

A

-35 Sequence - Consensus sequence 35bp left of transcription start site
Pribnow Box - Consensus about 10 bp upstream of transcription start site. It is the site of DNA melting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Elongation
Steps of Elongation

A

DNA Helix unwinds by RNA Polymerase
Synthesis of RNA begins
DNA-RNA hybrid helix is formed in 5’ → 3’ direction
Growing chain dissociates from DNA
DNA Helix rewinds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Elongation
Characteristics of RNA Polymerase activity

A

Does not need primer
Has no exonuclease activity

17
Q

Elongation
Carried out by Core Enzyme or Holoenzyme?

A

Core Enzyme

18
Q

Elongation
Errors during elongation

A

RNA Polymerase pauses, backtracks, cleaves transcripts and restarts

19
Q

Termination
Where does Elongation continue until?

A

Until Termination Signal is reached

20
Q

Termination
Which protein is responsible for Termination?

A

Rho Factor

21
Q

Termination
Rho Factor function

A

Separates DNA-RNA hybrid

22
Q

What does pro RNA do after Termination?

A

Self-complementary pro RNA causes hairpin structure

23
Q

Antibiotics inhibiting RNA Polymerase

A

Rifampicin

MD2S3: Molecular Biology (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions