Transcription And RNA Processing Part 1

Question 1

RNA polymerase I

Answer 1

-rRNA 18S, 28S, 5.8S

-

Question 2

RNA Polymerase II

Answer 2

• mRNA, miRNA, snRNA

- polyadenylated

Question 3

RNA Polymerase III

Answer 3

-tRNA, U6 snRNA, 5S rRNA

Question 4

TATAA

Answer 4

Where TBP binds

Question 5

TFIID

Answer 5

Contains TBP and TAFs, binds promoter

Question 6

TFIIB

Answer 6

Binds BRE and TBP

Question 7

TFIIH

Answer 7

Helicase activity and phosphorylates 5th serine of CTD of RNA polymerase II, leading to dissociation of Pol2 from preinitiation complex, initiating transcription

Question 8

rRNA processing occurs in

Answer 8

Nucleolus

Question 9

tRNA processing

Answer 9

RNAse P processes 5’ end,
RNAse cleaved 3’ end and 3’ end processed thru addition of CCA end (site of AA attachment)
-modifications at 10% of bases stabilize the hairpin loops

Question 10

rRNA processing

Answer 10

Cleavage of 45S unit into smaller

• links thru H bonds
• methylation of bases and sugars
• chemical conversions of bases