DNA Replication 1

Question 1

Acyclovir

Answer 1

Greater affinity for viral DNA polymerase than cellular, used for herpes

Question 2

Origins of replication

Answer 2

AT base rich.

Unique sites where replication begins

Question 3

AAA+ ATPase

Answer 3

DNAA binds to the origin of replication and dissociated the helical strands. Energy from ATP used to induce conformational change in DNAA which forces strands apart

Question 4

DNA kinase

Answer 4

Creates phosphodiester bonds by energy from ATP cleavage. Seals nicks in strand

Question 5

DNA polymerase

Answer 5

Strand elongation, require ssDNA as template and require RNA primer

Question 6

Helicase

Answer 6

Dissociation of two stands, unwinding them using energy from ATP

Question 7

Nucleases

Answer 7

Sever phosphodiester bonds of DNA backbone, endo or exo nuclease

Question 8

Primate

Answer 8

Synthesized short stretches of RNA complementary to template to serve as a primer for DNA polymerase

Question 9

Topoisomerase

Answer 9

Adjust supercoiling, inducing neg supercoiling. Has endonuclease and ligaments function. Type 1= one strand
Type II- cleave both strands

Question 10

Topoisomerase II poisons

Answer 10

Stabilize covalent DNA topoisomerase II complex, antitumor

Question 11

Catalytic inhibitors

Answer 11

Act on other steps in catalytic cycle. Antineoplastic, cardioprotectors, modulators, increase efficiency of other agents

Question 12

DNA polymerase II, IV and V

Answer 12

Repair functions in prokaryotes

Question 13

DNA polymerase I

Answer 13

DNA repair, removes primers in replication, 5 to 3 and 3 to 5 exonuclease activity

Question 14

DNA polymerase III

Answer 14

Synthesize both DNA lagging and leading strands, 3 to 5 exonuclease activity