*Transcription

Question 1

RNA polymerase moves in __ to __ direction on template strand

Answer 1

3’ - 5’

Question 2

nascent RNA grows __ - __

Answer 2

5’ - 3’

Question 3

Transcription initiation

Answer 3

RNA polymerase binds, has a sigma factor in prokaryotes

Question 4

Bacterial promoter has 2 basic elements:

Answer 4

1. consensus sequence -35bp upstream of initiation site

2. Pribnow box -10 bp upstream of initiation site

Question 5

Sigma factors

Answer 5

recognize different versions of promoter sequence in genes

Question 6

What takes transcription from basal to activated levels?

Answer 6

TAFS (TBP associating factors) stabilize TBP complex by linking to upstream enhancer binder proteins

Question 7

Which end of mRNA is capped with 7-me-guanosine?

Answer 7

5’

Question 8

Which end of mRNA is the poly-A tail added to?

Answer 8

3’

Question 9

mRNA splicing occurs _____ 5’ and 3’ modifications (cap and tail)

Answer 9

AFTER

Question 10

Nuclear Pore Complex

Answer 10

Huge, (~30 ribosomes) 8 fold symmetry (8 copies of nucleoporins) - mediates nuclear import and export

Question 11

Aly

Answer 11

Protein that is a component of Exon-Junction Complex (EJC)

Question 12

TAP

Answer 12

Protein that associates with Exon-Junction Complex (EJC) to drive it through the nuclear pore complex.

Question 13

Exon-Junction Complex

Answer 13

• a protein complex which forms on a pre-messenger RNA strand at the junction of two exons which have been joined together during RNA splicing.
• The spliceosome places it 24-30 NTs upstream of each exon/exon junction.