Transcription Flashcards
1
Q
mRNA is synthesised by which type of RNA polymerase?
A
RNA pol II
2
Q
Transcription requires which three things?
A
RNA polymerases, NTPs and DNA
3
Q
What are the 3 broad steps of transcription?
A
Initiation, elongation and termination
4
Q
Where do transcription factors bind to attract RNA polymerase for transcription?
A
The TATA box
5
Q
Growth of the mRNA chain occurs in which direction?
A
5’->3’
6
Q
What enzyme catalyses polyadenylation?
A
polyA polymerase
7
Q
What type of cap is added to the mRNA?
A
A methyl-guanine “cap”
8
Q
What is removed from the premRNA during splicing?
A
Introns
9
Q
What is a gene?
A
A gene is a stretch of DNA found on a chromosomal locus. It is a unit of inheritance and transcription