Enzyme activity: kinetics and inhibition

Question 1

What is V0?

Answer 1

The initial rate of reaction

Question 2

Reaction velocity against substrate concentration gives which sort of curve?

Answer 2

A hyperbolic curve

Question 3

What is Vmax?

Answer 3

The maximal rate when all enzyme active sites are saturated with substrate

Question 4

What is Km?

Answer 4

Km is the substrate concentration that gives half the maximal velocity

Question 5

A lineweaver-burk plot is just the reciprocal of enzyme rate graphs. True or false?

Answer 5

True

Question 6

Competitive inhibitors bind to a site other than the active site. True or false?

Answer 6

False

Question 7

What is competitive inhibitors effects upon Km and Vmax?

Answer 7

Km- increases

Vmax is unaffected

Question 8

What is non competitive inhibitors effects upon Km and Vmax?

Answer 8

Km is unaffected and Vmax decreases

Question 9

What is the active site of the enzyme?

Answer 9

It Is the place where substrates bind and where the chemical reaction occurs

Question 10

What is allosteric regulation?

Answer 10

Allosteric regulation is the regulation of a protein by binding an effector molecule at a site other than the enzymes active site