Transcription Flashcards
What happens during transcription?
The double-stranded DNA is used to make a single-stranded template that is a copy of the gene. This copy is made of mRNA.
Step one of transcription
Sections of double-stranded DNA are unravelled and hydrogen bonds between complementary base pairs on the coding and non-coding strands are broken by DNA Helicase
Step two of transcription
RNA polymerase binds to the promoter region of the DNA
Step three of transcription
Free RNA nucleotides in the nucleus are able to form weak hydrogen bonds with complementary base pairs on the non-coding strand
Step 4 of transcription
RNA polymerase catalyses condensation reactions to form phosphodiester bonds between the RNA nucleotides to make a strand of mRNA
Step five of transcription
Pre-mRNA is spliced to remove non-coding introns from the gene