Transcription Flashcards
1
Q
what does transcription do?
A
DNA becomes RNA (3 types)
2
Q
where?
A
nucleus
3
Q
4 key points
A
- anti parallel
- complementary
- mRNA from 5 to 3
- U instead of T
4
Q
Types of RNA
A
- mRNA (protein synthesis)
- rRNA (ribosomes)
- tRNA (transfer AA from cytoplasm to ribosome)
5
Q
Initiation
A
- RNA poly finds the two promoter sequences
- one of them is TATA box. 2nd one is for extra insurance
- RNA attaches to template strand (3 to 5)
6
Q
Elongation
A
- RNA poly and other proteins make transcription complex which uwinds DNA
- nucleotides pair with template strand
- DNA reanneals
7
Q
Termination
A
- RNA poly meets terminator sequence which is 2 terminators in a row.
- mRNA separates from RNA poly which detaches from DNA
8
Q
Post transcription proccess?
A
- mRNA has a 5’ cap added on which will help start translation later
- poly A tail added which will help elongate life of mRNA. each time a protein is synthesized from mRNA, it shortens a bit
- spliceosomes/snRP’s remove introns
