Transaminases

Question 1

L-alanine byproduct

Answer 1

Pyruvate

Question 2

This serves as the coenzyme of ALT/ AST

Answer 2

P5P (activated form of vit. B6)

Question 3

L-aspartate byproduct

Answer 3

Oxaloacetate

Question 4

True or False
Chronic alcohol drinkers have low levels of ALT/AST activities

Answer 4

True

Question 5

AST in found in?

Answer 5

30% in cytoplasm, 70% in mitochondria

Question 6

Half-life of AST

Answer 6

Extramitochondrial - 17 hrs
Mitochondrial - 87 hours

Question 7

ALT, in liver cells, is found in?

Answer 7

Cytoplasm

Question 8

ALT half-life

Answer 8

47 hours

Question 9

Sources of AST

Answer 9

Heart, liver, skeletal muscle (high concentration)
Kidney pancreas, and erythrocytes (smaller amounts)

Question 10

Specimen requirements for testing AST

Answer 10

Serum free from hemolysis (kasi may small amounts of AST sa erythrocytes)
Turbidity and icteresia (affects the assay)
Stable for 1 week if refrigerated

Question 11

In measuring oxaloacetate (AST byproduct), what method uses 2 enzymes to

Answer 11

Karmen method
(2 enzymes: AST & MDH)

Question 12

It is used as an indicator reagent of Karmen Method

Answer 12

Malate dehydrogenase

Question 13

What is the principle of Karmen method?

Answer 13

It measures the decrease in absorbance of NAD at 340 nm
(Oxakoacetate + NAD + H —MDH—> malate + NAD)

Question 14

What color is seen when oxaloacetate is combine with 2,4 DNPH?

Answer 14

Reddish brown
(Measures at 505 nm)

Question 15

Reference range of AST

Answer 15

8-40 U/L OR 5-30 U/L

Question 16

Reference range of AST

Answer 16

8-40 U/L OR 5-30 U/L