The Gonadal Hormones Flashcards
Test principles used in steroid hormone analysis
Colorimetric method
Both test for steroid hormone analysis uses colorimetric (17-KS.and Urin
Zimmerman Reaction in Urine 17-Ketosteroids (17-KS)
what is the color reaction in Zimmerman reaction?
17-KS when treated with metadinitrobenzene in alcohol alkali produces a
Red-purple
Urine 17-Hydrocorticosteroids
What are the 2 methods used to test for Urine 17-Hydrocorticosteroids
please observe the proper use of capitalization of letters and punctuation marks, as needed.
Porter-Silber Method and Zimmerman procedure
Estimates 11-deooxycortisol, Cortisol, Cortisone in urine.
Porter Silber Method
what is the color reaction in Porter-Silber Method?
Reagent: 2,4-dinitrophenylhydrazine and H2SO4
yellow
measured at 410nm
Zimmerman procedure/reaction in Urine 17-Hydrocorticosteroids
Aside from 11-deoxycortisol, Cortisol, Cortisone; what other reagents are used in Zimmerman procedure?
17-hydroxyprogesterone and 17-hydroxypregnenolone
thesea re used to determine pregnanetriol and metabolites.
Gonadal Hormones diseases
Pathophysiology: Abnormal balance of androgen hormones in women, causing excessive hair growths in areas like moutha and chin.
Hirsutism
Gonadal Hormones
Primary male growth hormone, which stimulates the production of male gonads
Androgen
Androgen
What are the 2 main androgens?
testosterone and androstenedione
Genital ducts
As androgen stimulates the developing genital ducts, it produces which duct?
mention both the present name and it’s (former name) inside parenthesis
follow proper letter capitalization; for special characters, just use the normal letters)
mesonephric (Wolffian ducts)
Genital ducts
Without any androgen stimulation the developing genital ducts develops into which duct?
mention both the present name and it’s (former name) inside parenthesis
follow proper letter capitalization; for special characters, just use the normal letters)
paramesonephric (Mullerian ducts)
No androgen stimulation results in forming female geitalia by default.
Gonadal Hormones Diseases
Pathophysiology: Hyperandrogenism, ovulatory dysfunction, aberrant relaeasing of GnRH, insulin resistance which may cause infertility, hirstutism, chronic anovulation, glucose intolerance, hyperlipidemia, hypertension, weight gain
Polycystic Ovary Syndrome (PCOS)
Gonadal hormones
2 pituitary hormones which controls testosteron secretion
answer using short term
FSH and LH
Gonadal hormones
which cells in pituitary gland prodces FSH and LH?
gonadotrophs
Organs
which organ produceh GnRH hormone?
Hypothalamus
Gonadal hormones
on which cells does FSH primarily acts upon?
germinal stem cells
gonadal hormones
on which cells does LH primarily acts upon?
please observe the proper capitalization of letters for proper nouns.
Leydig cells
In PCOS investigation one test that is used is free testosterone.
what protein attaches to the active form of testosterone that is measured in the said test?
albumin
Testosterone follows a circadian fashion
what is the peak time of testosterone production
8am
Testosterone follows a circadian fashion
what is the lowest time of testosterone production
12am-4am
PCOS investigation lab results:
What is the expected lab result for PCOS patients:
1. Free testosterone
2. SHBG
3. FSH
4. LH
5. FBG
6. Insulin
7. Lipid levels
- increase
- decrease
- increase
- increase
- increase
- increase
- increase
hormones
this globulin binds testosterone and estrogen
Sex hormone bindign globulin
hormones
most potent form of estrogen, which assists in FSH and LH regulation to develop endometrium with progesterone
short name (full name)
E2 (Estradiol)
hormone
Major estrogen produced during pregnancy
E3 (Estriol)
hormone
which form of estrogen is an indicator of ovarian function
short name
E2
hormones
peak time of FSH and LH release
mid gestation
hormone
FSH and LH levels at birth
low
Gonadal hormone disease
common cause of secondary Amenorrhea