Toxoplasma Flashcards

1
Q

a protozoan parasite, phylum Apicomplexan and often referred to as a coccidian

A

Toxoplasma

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2
Q

who does Toxoplasma infect?

A

infects most species of warm-blooded animals, including humans, but only multiply sexually in cats
- infect 1/3 of world popl’n = mostly asymptomatic

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3
Q

Toxoplasma lives an obligate intracell lifestyle and has a unique ability to…

A

invade host cells and evade immune system

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4
Q

two basic morphologies of toxoplasma

A

trophozoites and cysts
both extraintestinal and seen in several tissues

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5
Q

toxoplasma trophozoites

A

two gens: tachyzoites (fast multiplying), bradyzoites (inert or slow multiplying = at this point, huddle together to form cysts)

shape: crescentic, single nucleus; asexual multiplication via endodyogeny (internal budding)

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6
Q

T. gondii lytic cycle

A

process of invasion and destruction of host cell by indiv Toxoplasma trophozoites
- tachyzoite = initial invader = parasitophorous vacuole is thin membrane that protects it
- keep multiplying until host cell lysis
- parasite egress = frees out daughter trophozoites to start new cycle of destruction in neighbouring host cells

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7
Q

toxoplasma cysts

A
  • also extraintestinal AK tissue cyst)
  • resting/chronic/diagnostic stage
  • thick wall (protection from immune system, digestive enzymes, inflammatory rxn)
  • result of asexual multiplication
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8
Q

intestinal form of toxoplasma parasite

A

seen in cats only due to sexual repro (gamogony = jejunal epithelium of cats)

oocyst with two sporocysts containing 4 sporozoites; each protected with tough but flexible wall

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9
Q

pathogenesis of toxoplasmosis

A
  • intracellular parasite of both phagocytic & non-phagocytic cell, characteristically invading only nucleated cells !!!!
  • cell invasion facilitated by the apical complex/drill- like conoid, micronemes, rhoptries, and dense granules
  • survival assured by creation of protective parasitophorous vacuole (comes from repurposing host membrane) inside host cell
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10
Q

conoid

A
  • central part of apical complex
  • hundreds of fibers in a spiral; like drill
  • can retract, extend, rotate
  • can secrete compounds that aid in attachment and penetration of host cell
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11
Q

main secretory organelles that participate in cell entry for toxoplasma

A

micronemes, thoptries, and dense granules

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12
Q

micronemes

A
  • apical complex; toxoplasma
  • release very early in
  • host cell binding and gliding motility
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13
Q

T or F. Apicala complexes are unique to Toxoplasma gondii

A

F! seen in apicomplexans

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14
Q

rhoptries proteins

A
  • Toxoplasma
  • released early during invasion
  • can be detected in lumen of newly generated paratoph vacuole
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15
Q

dense granules

A
  • Toxoplasma
  • released after during/after formation of paratoph vacuoles
  • often modify vacuoles environment
    = aiding intracell survival and replication of parasite
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16
Q

organs affected by toxoplasma

A

retina (eyes)
brain
lymph nodes
liver

17
Q

T or F. Transmission across placenta in a pregnant woman to fetus can only occur if toxoplasma was ingested after conception

A

T!

18
Q

sites of invasion of Toxoplasma

A
  • brain = behavioural changes
  • eye (immunologically sequestered site, just like brain)
  • lymph nodes
  • cardiac or skeletal muscle
19
Q

Two phases of Toxoplasma during human stage

A

Early (acute) phase: - tachyzoites invading cells
- cell destruction/necrosis
- mononuclear cell inflammation
- blood vessel blockage

Late (chronic) phase:
- bradyzoites/tissue cysts
- latency/immunological evasion
- hypersensitivity

Reactivation of chronic infection after being latent

20
Q

clinical picture of toxoplasmosis

A
  • acute:
    asymptomatic = 90%
    lymphadenopathy
    infectious mono-like
    hepatitis with jaundice
    splenomegaly
    rash
    RARE: involvement on CNs and eye
  • chronic: ALWAYS asymptomatic (unless of course blind = residual effect)
21
Q

ocular toxoplasmosis

A

can be seen during ophthalmoscopic eye exam = dull area where hemorrhage occurred

22
Q

Neonatal Toxoplasmosis (2)

A

Transplacental transmission: acute toxoplasmosis in pregnancy

Congenital toxoplasmosis:
* abortion or stillbirth * CNS lesions = mental retardation
* eye involvement: blindness

23
Q

toxoplasmosis in pregnancy:risk of congenital infection

A

first trimester = 5%, 60% of offsprings show clinical signs

2nd trimester = 40%, and 25% of offsprings show clinical signs

third trimester = 70%, 10% of offsprings show clinical signs

24
Q

principal reservoir of toxoplasma

A

rodents

25
Q

how is toxoplasma transmitted?

A

ingestion
transfusion (not red cells) or transplantation
transplacentally

or.. reactivation of chronic infection

26
Q

epidemiology of toxoplasma

A

worldwide!
doesn’t kill ppl
humans, cats, lambs, swine, cattle, chickens

27
Q

lab diagnosis of toxoplasmosis

A
  1. Direct demonstration:
    - Giemsa stain
    - PAS (Periodic Acid-Schiff)
    - Fluorescent antibody stains
    - NAAT testing Nucleic Acid Amplification Tests); PCR on tissue samples and body fluids(including amniotic fluid)
    - Animal inoculation/tissue cultures (now rarely used)
  2. Serology :
    - Sabin Feldman dye test
    - Immunofluorescent assays (IFA)
    -Enzyme immunoassays (EIA) : IgG, IgM, other Ig classes
28
Q
A