Balantidium & Cryptosporidium Flashcards
first recognized in two humans with dysentery in 1857
Balantidium coli
only ciliated protozoan known to infect humans
B. coli
largets protozoan infecting humans and non-human primates
Balantidium
vehicle of Balantidium for most cases
water
T or F. Balantidiosis is a zoonotic disease
T
definite host of Balantidium
porcein
- asymptomatic in pigs and humans
- can be found wherever pigs are found
- human to human may occur but RARE
biology and life cycle of B. coli
- unicellular trophs covered in cilia (longitudinal wavelike motion); 30 to 150 um by 25-120um
> vestibule is anterior portion of mouth
> cytostome is the mouth
> cytophage = anus where waste is discharged
> macronucleus = shaped like large sausage; non-reproductive functions like feeding and metabolism
> micronucleus = small organ that sits below macronucleus; genetics and cell division - cyst
> transmissive stage (40 to 60 um in diameter)
> tough membrane (formed as trophs move down intestines
> can survive dessication & environmental stage
> survives best in human surroundings protected from sunlight
> bacteria and food particles and RBCs main food source
> no nuclear/cytoplasmic division
T or GF. Micronucleus of B. coli is easily seen on the microscope
F
where can B. coli cysts be seen?
in formed stool; macronucleus = thick double wall; transmittive stage
how does B. coli reproduce?
asexual = transverse fission; occurs for most ciliates
sexual = conjugation followed by binary fission
> role of macro- and micronucleus here
> during binary fission= exchange of micronuclear material; macro will disintegrate during conjugation; new one formed by micronucleus => binary fission will then occur
- two sizes of trophs (large and small) are formed during conjugation; never bw same sizes
simple life cycle of B. coli
- ingestion of contaminated food or water
- hatching of parasite
- gut invasion of parasite
- encystation of trophs
- excretion of cysts
B. coli = lesions formed due to ulcers in colon
forms flask shaped lesions in submucosa
= form clusters which are referred to as nides or nes
lumen dwellers
B. coli
- cecum and colon
gut wall invasion of B. coli produces this enzyme
hyaluronidase
- degrades intestinal tissue & digests hyaluronic acid = glue that holds mucosal epi cells together
- during invasion = bacterial and inflammatory rxn occur
- mucosa ulcers and subsurface abscesses form
- mucosa and submucosa are damaged
- muscular layer also affected =hemorrhage and necrosis
extraintestinal invasion of B. coli
- may include appendix
T or F. B. coli produces many toxins that help with pathogenesis
F! does not produce any known toxins
symptomatology of B. coli
- asymptomatic in humans and pigs
- diarrhea -> dysentery (nausea, vomiting, or headache)
- rarely appendicitis , UTI, vaginitis/cervicitis, peritonitis
B. coli reservoir
zoonotic disease
normal host (75% pigs)
also monkey, dog, cattle, sheep, rat
- high prevalence in poor rural areas such as Latin America, Philippines, etc.
how are humans affected by . coli
- fecal oral-route
- sewage sludge (human waste as fertilizer)
risk factors: pig farmers, slaughterhouse workers, vets, poor nutritional status, intestinal flora not normal, and also dependent on parasitic load, alcoholics, etc.
epidemics of B. coli
mental hospitals and prisons
B. coli diagnosis
stool microscopy (trophs more prominent than cysts (maybe difficult to ID; can be confused by other helminths))
tissue biopsy of colon
cultivation (grow between 20C to 40C)
prevention of B. coli
improved sanitation and hygiene for water and food supply
chlorine not effective against thick wall of B. coli cysts
prevent contamination of water source by pigs/human feces
treatment for B. coli
tetracycline
or metronidazole flagyl