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Biology > Topic 4 - Genetic information > Flashcards

Topic 4 - Genetic information Flashcards

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1
Q

What are the properties of eukaryotic DNA ?

A

DNA molecules are very long, linear and compacted into chromosomes by histoness.

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2
Q

What are properties of eukaryotic mitochondria and chloroplast DNA ?

DNA, Genes and Chromosomes

A

Shorter, circular DNA molecules with no histone proteins.

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3
Q

What are the properties of prokaryotic DNA ?

DNA, Genes and Chromosomes

A

DNA molecules (chromosomes) are circular and shorter with no histone proteins associated.

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4
Q

What is a gene ?

DNA, Genes and Chromosomes

A

A sequence of bases that code for an amino acid or a functional RNA.

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5
Q

What determines the order of amino acids in a polypeptide ?

DNA, Genes and Chromosomes

A

The order of bases in a gene.

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6
Q

What is a triplet ?

DNA, Genes and Chromosomes

A

3 bases in a gene which code for an amino acid.

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7
Q

What do introns code for ?

DNA, Genes and Chromosomes

A

Functional RNA
e.g. tRNA and rRNA.

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8
Q

What is a cell’s genome ?

DNA, Genes and Chromosomes

A

The complete set of genes in a cell.

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9
Q

What is a cell’s proteome ?

DNA, Genes and Chromosomes

A

The full range of proteins that the cell is able to produce.

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10
Q

How many genes code for 1 polypeptide ?

DNA, Genes and Chromosomes

A

One gene codes for one mRNA molecule, which codes for one polypeptide.

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11
Q

What are introns ?

DNA, Genes and Chromosomes

A

Sections of a gene that dont code for amino acids, instead they code for functional RNA.

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12
Q

What are exons ?

DNA, Genes and Chromosomes

A

All of the parts of a gene which code for a specific order of amino acids.

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13
Q

What happens to introns during protein synthesis ?

DNA, Genes and Chromosomes

A

They’re removed so they dont effect the order of amino acids.

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14
Q

What are non coding repeats ?

DNA, Genes and Chromosomes

A

regions of multiple repeats that do not code for amino acids. They are located between genes.

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15
Q

What are alleles ?

DNA, Genes and Chromosomes

A

Different forms of a gene

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16
Q

How do alleles form slightly different versions of the same polypeptide ?

DNA, Genes and Chromosomes

A

The order of bases in each allele is slightly different

17
Q

How many chromosomes does a eukaryotic nucleus have ?

DNA, Genes and Chromosomes

A

23 pairs, 46 in total

18
Q

What are homologous pairs of chromsomes ?

DNA, Genes and Chromosomes

A

pairs of matching chromosomes, they have the same genes but they could have different alleles. Alleles coding for the same charactersistic will be found at a same fixed potiton (locus) on each chromosome in the pair.
An example is the gene that determines blood type exists as one of 3 alleles, type O, A and B.

19
Q

What is a locus ?

DNA, Genes and Chromosomes

A

The same fixed postiton where alleles cpding for the same characteristsic are found.

20
Q

What is messsenger RNA ?

RNA and Protein Synthesis

A

It is made during transcription, it carries the genetic code from DNA to the ribosomes where it is used to make proteins during translation. mRNA is a single polynucleotide strand.

21
Q

What is a codon ?

RNA and protein synthesis

A

3 bases in a mRNA polynucleotide

22
Q

What is transfer RNA ?

RNA and Protein Synthesis

A

tRNA is involved in translation. It carries amino acids that are used to make porteins to the ribosomes. It is a songle polynucleotide strand thats folded into a clover shape. Hydrogen bonds between specific base pairs hold the molecules in this shape. Every tRNA molecule has a specific sequence of 3 bases at one end called an anticodon. The other end is an amino acid binding site.

23
Q

What is an anticodon ?

RNA and Protein Synthesis

A

a sequence of 3 specific bases at one end of a tRNA molecule.

24
Q

Name the first stage of protein synthesis

RNA and Protein Synthesis

A

Transcription

25
Q

Describe what happens during transcription

RNA and Protein Synthesis

A
  1. RNA polmerase attaches to the DNA molecule at the beginning of a gene.
  2. The hydogen bonds between the complimentary base pairs of the double helix are broken down by DNA helicase which is attached to RNA polymerase. This separates the DNA molecule, uncoiling, and exposing the bases.
  3. RNA polymerase lines up free RNA nucleotides alongside te exposed DNA bases on the template strand. Specific, complimentary base pairing occurs casuing a complimentary copy of the DNA strand to be made, however as it is RNA, uracil is in place of Thymine.
  4. RNA polymerase joins the nucleotides by phosphodiester bonds and an mRNA molecule is formed.
  5. When RNA polymerase reaches a stop signal, the mRNA detaches from the DNA.
  6. pre- mRNA is spliced to remove the introns and connect exons, forming an mRNA stranf which leaves the nuceus through a nuclear pore.
26
Q

What is the last stage of protein synthesis?

RNA and Protein Synthesis

A

Translation

27
Q

Describe what happens during translation.

RNA and Protein Synthesis

A
  1. mRNA attaches to a ribosome.
  2. A tRNA molecule with an anticodon complimentary to the mRNA’s first codon attaches itself to the mrna by specific complimentary base pairing.
  3. This tRNA molecule contains an amino acid. ATP provides energy needed for the bond between the tRNA and the amino acid to form.
  4. A second tRNA molecule attaches to the second codon in the same way.
  5. The two amino acids join by peptide bonds and the first tRNA molecule moves away, leaving the amino acid behind. This process continues until there is a stop signal on the mrna strand, producing a polypeptide chin.
  6. The polypeptide chain moves away from the ribosome.
28
Q

Where does translation take place ?

RNA and protein synthesis

A

Ribosome

29
Q

Where does transcription take place ?

RNA and protein synthesis

A

Nucleus

30
Q

What is the genetic code ?

The genetic code

A

The sequence of codons in mRNA which code for specific amino acids.

31
Q

What three properties does the genetic code have ?

The genetic code

A

It is non overlapping - codons do not share bases
It is degenerate - some amino acids can be coded for by more than one codon
It is universal - the same codon will code for the same specific amino acid in all living things

Biology (14 decks)

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