topic 4 Flashcards
what does it mean if the genetic code is degenerate?
more possible combinations of triplets than amino acids
what does it mean if the genetic code is universal?
the same triplet of bases codes for the same amino acids in all organisms
what does it mean if the genetic code is non overlapping?
each base is only part of one triplet
what is a triplet made up of?
3 amino acids
what is a gene?
base sequence of DNA that codes for the amino acid sequence of a polypeptide
what is a start codon?
at the start of every gene to initiate translation
what is a stop codon?
at the end of every gene, causes ribosomes to detach and stop translation
what are introns?
non coding sections of DNA, only in eukaryotes, spliced out of mRNA
what are exons?
sections of DNA that code for amino acids
what is a genome?
an organism’s complete set of DNA which never changes
what is a proteome?
an organism’s full range of proteins which always changes
what is a locus?
the location of a gene on a chromosome
what is an allele?
an alternative form of a gene
what is a chromosome?
where DNA is stored
how many pairs of chromosomes are there in humans?
23
where is DNA stored?
in the nucleus of eukaryotic cells, and is circular in prokaryotic cells
what is the purpose of transcription?
a gene is copied into mRNA which can carry the genetic code to a ribosome so a protein can be made
what are the stages of transcription?
1) DNA helix unwinds to expose the bases and act as a template
2) a chain of DNA acts as a template
3) catalysed by DNA helicase
4) which breaks the hydrogen bonds between bases
5) free mRNA nucleotides in the nucleus align with exposed complementary bases
6) RNA polymerase bonds RNA nucleotides by phosphodiester bonds
7) pre mRNA is spliced to form mRNA
8) mRNA moves out of the nuclear pore and attaches to a ribosome in the cytoplasm
what is the purpose of translation?
to produce a new protein
what happens in translation?
mRNA joins with a ribosome, the tRNA brings along the specific amino acid
what are the stages of translation?
1) mRNA attaches to a ribosome at the start codon
2) tRNA has an anticodon complementary to the mRNA codon
3) tRNA brings along a specific amino acid
4) amino acids are joined by peptide bonds using ATP
5) tRNA is released
6) ribosome moves along mRNA to form a polypeptide
7) it detaches at the stop codon
8) and enters the golgi for folding and modification
what is RNA made of?
ribose, nitrogenous base, phosphate group
what are the bases for RNA?
adenine, guanine, cytosine, uracil
what is the structure of RNA?
a short, single polynucleotide strand
what is the job of RNA?
to copy and transfer the genetic code from the DNA in the nucleus to the ribosomes
what is mRNA?
a copy of a gene from DNA
where is mRNA created?
in the nucleus
where does mRNA go?
made in nucleus, leaves to carry a copy of the genetic code to a ribosome in the cytoplasm