Topic 3 Flashcards
2 ml plasma + 0.2ml adsorbing reagent, centri at 3000rpm for 3 mins
Adsorbed normal plasma
1 part 0.109M sodium citrate and 9 parts blood
Centri t 1500 for 5 mins
Plasma routine technique
Collect plasma in the usual manner
Incubate at 37C for 24hrs, store in aliquot at -20
Aged normal plasma
Collect serum in nirmal
Manner
Allow to stand at room temp for 24 hrs
Divide in aliquot and freeze
Aged normal serum
Commonly used in blood coagulation
Platelet poor plasma
1:10 dilution of whole blood and anticoagulant
Centri at 3000rpm for 30 mins
Platelet poor plasma
1:10 dilution of blood an anticoagulant
Centri at 1500rpm for 5 mins
Platelet rich plasma
Universal test for platelet function and for diagnosis of vWD
Bleeding time
The result is directly affected by platelet count and the platelet’s ability to form hemostatic plug
Bleeding time
Factors that will affect bleeding time (3)
Elasticity of the cut tissue
Ability o blood vessels yo constrict and retract
Mechanical and chemical action of platelets in the formation of hemostatic plug
Bleeding time method
NV 1-3 mins
Duke’s method
Bleeding time method
NV 2 1/2-7 mins
Modified Ivy’s method
A modification of ivy’s method
Template method
Use of special template to ensure that a cut is exactly 9mm long and the puncture is 1 mm into skin
Template method
Sphygmomanometer is placed on the upper arm an inflated to 40 mmHg
Ivy’s method
Bleeding time method
Incision is 5 mm long and 1mm deep
Similar to template method except for the use of an instrument to produce standard incision
Simplate II
Bleeding time method
Asses the effect of a standard dose of aspirin on the duke’s time
Aspirin tolerance test
Bleeding time method
Similar with adelson crosby, only it used the ear lobe as the spit of puncture
MacFarlane’s method
Both method involve immersion of the warmed wounded finger in sterile NSS at 37C until bleeding stops
Coply Lalitch method
Adelson-Crosby method
Method of bleeding time determination
NV 160-340 secs
Coply Lalitch method
Adelson-Crosby method
Blood platelet reduced
Conditions (3)
Thrombocytopenic purpura
Acute leukemia
Aplastic anemia
Injury of capillary wall
Conditions (3)
Scurvy
Toxins (infection, chemical, snake venom)
Allergy
Prothrombin deficiency
Condition (2)
- destructive disease of the liver with hemorrhagic tendencies
- hemolytic disease of the newborn
Enumeration o platelets
(Platelet count)
Less than 100000/uL
Abnormally low
REJECT
Enumeration o platelets
(Platelet count)
30k-50k/uL
Bleeding possible with trauma
Enumeration o platelets
(Platelet count)
Less than 30k/uL
Spontaneous bleeding possible
Enumeration o platelets
(Platelet count)
Less than 5000/uL
Severe spontaneous bleeding
Normal value of platelets
150k-450k/uL
Enumeration o platelets
Platelet count
Reject
Enumeration o platelets
Platelet count
Reject
Removal of factor 2, 7,9,10 by the use of Al(OH)3, BaSo4, Ca3(PO4)2
Adsorption
