Topic 3

Question 1

2 ml plasma + 0.2ml adsorbing reagent, centri at 3000rpm for 3 mins

Answer 1

Adsorbed normal plasma

Question 2

1 part 0.109M sodium citrate and 9 parts blood

Centri t 1500 for 5 mins

Answer 2

Plasma routine technique

Question 3

Collect plasma in the usual manner

Incubate at 37C for 24hrs, store in aliquot at -20

Answer 3

Aged normal plasma

Question 4

Collect serum in nirmal
Manner
Allow to stand at room temp for 24 hrs
Divide in aliquot and freeze

Answer 4

Aged normal serum

Question 5

Commonly used in blood coagulation

Answer 5

Platelet poor plasma

Question 6

1:10 dilution of whole blood and anticoagulant

Centri at 3000rpm for 30 mins

Answer 6

Platelet poor plasma

Question 7

1:10 dilution of blood an anticoagulant

Centri at 1500rpm for 5 mins

Answer 7

Platelet rich plasma

Question 8

Universal test for platelet function and for diagnosis of vWD

Answer 8

Bleeding time

Question 9

The result is directly affected by platelet count and the platelet’s ability to form hemostatic plug

Answer 9

Bleeding time

Question 10

Factors that will affect bleeding time (3)

Answer 10

Elasticity of the cut tissue
Ability o blood vessels yo constrict and retract
Mechanical and chemical action of platelets in the formation of hemostatic plug

Question 11

Bleeding time method

NV 1-3 mins

Answer 11

Duke’s method

Question 12

Bleeding time method

NV 2 1/2-7 mins

Answer 12

Modified Ivy’s method

Question 13

A modification of ivy’s method

Answer 13

Template method

Question 14

Use of special template to ensure that a cut is exactly 9mm long and the puncture is 1 mm into skin

Answer 14

Template method

Question 15

Sphygmomanometer is placed on the upper arm an inflated to 40 mmHg

Answer 15

Ivy’s method

Question 16

Bleeding time method
Incision is 5 mm long and 1mm deep
Similar to template method except for the use of an instrument to produce standard incision

Answer 16

Simplate II

Question 17

Bleeding time method

Asses the effect of a standard dose of aspirin on the duke’s time

Answer 17

Aspirin tolerance test

Question 18

Bleeding time method

Similar with adelson crosby, only it used the ear lobe as the spit of puncture

Answer 18

MacFarlane’s method

Question 19

Both method involve immersion of the warmed wounded finger in sterile NSS at 37C until bleeding stops

Answer 19

Coply Lalitch method

Adelson-Crosby method

Question 20

Method of bleeding time determination

NV 160-340 secs

Answer 20

Coply Lalitch method

Adelson-Crosby method

Question 21

Blood platelet reduced

Conditions (3)

Answer 21

Thrombocytopenic purpura
Acute leukemia
Aplastic anemia

Question 22

Injury of capillary wall

Conditions (3)

Answer 22

Scurvy
Toxins (infection, chemical, snake venom)
Allergy

Question 23

Prothrombin deficiency

Condition (2)

Answer 23

• destructive disease of the liver with hemorrhagic tendencies
• hemolytic disease of the newborn

Question 24

Enumeration o platelets
(Platelet count)

Less than 100000/uL

Answer 24

Abnormally low

REJECT

Question 25

Enumeration o platelets
(Platelet count)
30k-50k/uL

Answer 25

Bleeding possible with trauma

Question 26

Enumeration o platelets
(Platelet count)
Less than 30k/uL

Answer 26

Spontaneous bleeding possible

Question 27

Enumeration o platelets
(Platelet count)
Less than 5000/uL

Answer 27

Severe spontaneous bleeding

Question 28

Normal value of platelets

Answer 28

150k-450k/uL

Question 29

Enumeration o platelets

Platelet count

Answer 29

Reject

Question 30

Enumeration o platelets

Platelet count

Answer 30

Reject

Question 31

Removal of factor 2, 7,9,10 by the use of Al(OH)3, BaSo4, Ca3(PO4)2

Answer 31

Adsorption