Topic 2 - cell structure Flashcards
What are the 3 different types of microscopy
Light microscope
Scanning electron microscope
Transmission electron microscope
What mediums do each microscopes use
Light microscope - light beam
Scanning electron microscope - electron beam
Transmission electron microscope - electron beam
What dimensions do each microscopes use
Light microscope - 2D
Scanning electron microscope - 3D
Transmission electron microscope-2D
What is the max magnification of each microscope
Light microscope - x1,500
Scanning electron microscope - x200,000
Transmission electron microscope - x2,000,000
What is the max resolution of each microscope
Light microscope - 200nm
Scanning electron microscope - 20nm
Transmission electron microscope - 0.1nm
What is magnification and state its equation
Magnification is how much bigger the image is compared to the original object viewed with the naked eye
Magnification = (size of image)/(size of object)
What is resolution
Resolution is how well a microscope distinguishes between two points that are close together.
To prepare slides for light microscopy, the specimen must be: (4 marks)
- Dehydrated
- Embedded in wax and sectioned
- Stained
- Mounted
Why is staining used when preparing light microscope
Staining is used to increase contrast in a specimen, in order to observe transparent and colourless structures.
How does staining work when preparing microscope slides
Stains work by binding to different types of molecules or cell structures. This allows different components of cells to be identified, and is known as differential staining
What are eukaryotic cells and 3 examples of eukaryotic groups
Eukaryotic cells are those cells that contain a nucleus and organelles enclosed by a plasma membrane (membrane bound organelles)
Includes animals, plants and fungal cells
Function of cell surface membrane
- Controls passage of entry of substance into the cell
- Site of cell communication via receptors
Function of the nucleus
- Stores DNA
- Nuclear pores allow mRNA & ribosomes to pass through
Function of the mitochondria
- carry out aerobic respiration to produce ATP
Function of lysosomes
Contains digestive enzymes to break down pathogens, old organelles, cells & food molecules
Function of ribosomes
Site of protein synthesis