Topic 2

Question 1

Beetroot - IV, DV and CV

Answer 1

IV - temp of water
DV - Absorbance
CV - volume of water, size of beetroot, type, time in water

Question 2

Beetroot method

Answer 2

Wash beetroot and cut into 1cm length and leave a night
Blot
Place in boiling tube and place in water bath
Leave for 10mins
Measure absorbance with colorimeter
Repeat 5 times per temp

Question 3

Enzyme IV,CV,DV

Answer 3

IV - conc of enzyme
DV - time taken for enzyme to break down substrate
CV - temp, volume of enzyme, of substrate, conc of substrate, pH

Question 4

Enzyme method

Answer 4

Make conc of protease solution (0-0.5)
Pipette 2cm of protein solution into cuvette
Measure absorbance of control
Measure out 2cm off protease solution into cuvette and immediately put in colorimeter
Measure absorbance every 30sec for 5mins