Topic 2 Flashcards

1
Q

Difference between prokaryotes and eukaryotes (2)

A
  1. Prokaryotes are smaller in size (bacteria cell size roughly equivalent to Mitochondria)
  2. Eukaryotes have membrane bound organelles that compartmentalize cells. Prokaryotes have cell wall/cell membrane –> compartmentalization is closed off.
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2
Q

Most bacteria cell are _______ micro-metres in length.

A

0.5 to 10

(Largest Prokaryote: Epulopiscium fishelsoni
Smallest Prokaryote: Mycoplasma pneumonia)

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3
Q

Why does surface area to volume ratio matter in terms of cell size and cell growth?

A

SA/V ratio affects how quickly cells exchange nutrients and waste into their environment. Small cells can grow/reproduce more quickly–> use less time/energy to replicate cells.

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4
Q

Shape of prokaryotes are determined by what factors? (4)

A

In no particular order:

  1. cell wall structure
  2. cell growth
  3. division mechanisms
  4. cell differentiation
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5
Q

Know your morphology vocab kidzz! (6 important terms)

A
  1. Coccus (sphere)
  2. Rod
  3. Spirillium (worm-like)
  4. Spirochete (S-shape/wavy hair shape)
  5. Stalk and Hypha (look like ladles)
  6. Filamentous
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6
Q

Name three types of Cocci: spheres and examples!!

A
  1. Staphylococci: cluster of cocci –> ex: Staphyloccus aureus, in human microbiota and opportunistic pathogen
  2. Dippococci: pairs of cocci –> ex: Neisseria gonorrhoeae, STD gonorrhea
  3. Streptococcus: chains of cocci –> ex: Streptococcus pyogenes, strep throat

Note: You can also have a single free-floating sphere —> ‘coccus’

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7
Q

Name Bacilli rod example:

A

Salmonella enterica –> food positioning and can cause typhoid fever

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8
Q

Example of Vibrio: Comma shaped:

hint: Neha’s pun ;)

A

Vibrio cholerae; human pathogen that can cause diarrhea and hydration

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9
Q

Name example of Helical shape:

Spring shaped

A

Helicobacter pylori; human stomach, cause of stomach ulcers, stomach cancers

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10
Q

Example of Spirochetes (Long spirals):

A

Borrelia burgdorferi; bacteria pathogen causing Lyme diseases by ticks

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11
Q

Example of Appendaged/Budding:

A

Caulobacter crescentus; study bacterial cell cycle, asymmetric cell division

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12
Q

Example of Filamentous Shape:

Noodles shaped!

A

Chloroflexus aurantiacus; photosynthetic bacteria don’t produce oxygen

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13
Q

How can cell shape affect aspects of day to day life? (5)

A

o Nutrient access/uptake (surface:volume ratio)
o Motility
o Attachment to surfaces
o Formation of biofilms
o Interactions with other microbes and/or eukaryotic host cells

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14
Q

Monomorphic vs Pleomorphic:

hint: mono means single; p stands for pleural

A

Monomorphic: adopt one shape; observed in most pure cultures of bacteria
Pleomorphic: multiple different morphologies for same bacterium, adopt multiple morphologies

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15
Q

What causes different morphologies/changes of morphology? (3)

A

o Differentiation into different cell types or spore formation –> cell program change
o Altered morphology in response to environmental stress
o Altered morphology due to mutation

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16
Q

What’s special about Arthobacter crystallopoietes?

A

Its pleomorphic!
Rod shapes during fast/logarthmic growth
Coccus during slow/ no growth

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17
Q

Major structures of the Cell Envelope: (4)

A
  1. Cytoplasmic membrane
  2. cell wall
  3. outer membrane
  4. S-layers
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18
Q

Roles of the Cell Envelope: (4 main ones)

A

o Maintains barrier with environment
o Protects cell from stress
o Allows transport of nutrients into cell and waste out of cell
o Energy conservation/production

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19
Q

Difference between Gram-negative and Gram-positive cell envelope?

A

Gram positive:
thick cell wall, no outer membrane, different/smaller periplasmic space

Gram negative: (think of a sandwich - has 2 membranes)
thin cell wall, outer membrane, has another cell membrane, periplasmic space b/w the two membrances

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20
Q

Explain the three functions of cytoplasmic membrane:

A
  1. Permeability barrier: prevents and functions as a gateway for transport of nutrients, waste in/out of cell
  2. Protein anchor: site of proteins that participate in transport, chemotaxis, bioenergetics
  3. Energy conservation: Site of generation and dissipation of proton motive force ( this basically saying that it helps pump photons against the energy/photon gradient)
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21
Q

Hydrophilic vs Hydrophobic:

A

Hydrophilic: “water loving” molecules; Ionic
and/or polar.
Hydrophobic: “water fearing” molecules; nonpolar.

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22
Q

Which parts of the cytoplasmic membrane are hydrophilic and hydrophobic?

A

Hydrophilic: Backbone –> glycerol and phosphate (is conserved in both euk. and prok.)
Hydrophobic: Fatty Acid Tails –> E.g. unsaturated
fatty acids (contain double bonds = kinks) increase fluidity (decrease rigidity) of membrane.

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23
Q

What are the three proteins found in the cytoplasmic membrane?

A
  1. Peripheral membrane proteins –> only on one side of the membrane
  2. Integral membrane proteins (embedded in membrane)
  3. Transmembrane proteins (are
    integral membrane proteins that pass all the way through membrane) –> runs from one side to another
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24
Q

Why does it mean when the Cytoplasmic membrane have “two faces”?

A

One side of cytoplasmic membrane faces the cytoplasm and the other faces outward (
periplasmic face).

Specific Topolgies!

2 faces of the cytoplasmic membrane are identical in respect to the phospholipids, but not identical cause the proteins in different direction make domains face different from one another.

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25
Q

Function of Cell Wall?

A
  • prevent cells from bursting due to osmotic
    pressure
    -cell shape, rigidity
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26
Q

What is peptidoglycan?

A

lattice-like structure formed from

chains of glycans linked together by peptide bridges

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27
Q

Glycan Chains in Pepidoglycan? (MEMORIZE)

A

N-acetylglucosamine (GlcNAc, NAG) &
N-acetylmuramic acid (MurNAc, NAM)
connected by β(1,4) linkage (glycosidic
bond)

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28
Q

Peptide Bonds in Peptidoglycan’s? (MEMORIZE THE IMAGE ON SLIDE - MY INSTINCT SAYS IT MAY BE ON THE EXAM)

A

• Sequence can vary
• D-isomers: amino acids; L-isomers: proteins
• Crosslinks between position 3 (diaminopimelic
acid “DAP” – can be a lysine) and
position 4 (D-alanine)

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29
Q

Gram negative bacteria:

A

~1-3 peptidoglycan layers

~2-7 nm

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30
Q

Purpose of Interbridges in Gram + bacteria:

A
connect
peptidoglycan layers (thicker than Gram - bacteria)
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31
Q

Purpose of Teichoic Acids in Gram + bacteria:

A

o Provide cell strength (ionic
interactions between metal ions)
o Help trap metal ions ex: Mg2+
o Barrier & attachment functions

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32
Q

What is Teichoic Acid?

A

Long polymers comprised of glycerol phosphate or ribitol phosphate with attached D-glucose and/or D-alanine

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33
Q

Role of Wall-associated proteins in Gram + bacteria:

A

cell adhesion

Ps: proteins can take part in covalent or non-covalent bonds with the cell wall

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34
Q

Gram staining influence on Gram positive bacteria?

A

thick layer of peptidoglycan is dehydrated – pores close and prevent escape of crystal violet dye – cells are stained purple

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35
Q

Gram staining influence on Gram negative bacteria?

A

decolorizing agent degrades outer membrane, thin/porous peptidoglycan layer does not retain purple stain. Cells appear pink due to safranin counterstain

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36
Q

Whats special about Mycoplasma pneumoniae?

A

Lacks cell wall due to minimal osmotic pressure in environment & has strong cell membrane

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37
Q

Three parts of Lipopolysaccharides (LPS)?

A
  1. lipid A (within membrane)
  2. core polysaccharide (sugar subunits connected to one another)
  3. O-specific polysaccharide (outermost component)
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38
Q

What’s lipid A?

A
  • Hydrophobic tails anchor in outer membrane
  • contains endotoxins –> immune system detects lipid A and sends message that bacteria was here

Note: mostly conserved from species to species

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39
Q

What’s O-specific polysaccharide?

A

o Polysaccharide comprised of diverse sugar subunits connected and branched in different ways
o Repeating combination of sugars with variable numbers of repeats

Note: it’s highly variable even within species!

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40
Q

Braun’s lipoproteins?

A

connect the outer membrane to cell wall

  • produced by ribosome attached to lipids
  • VERY abundant in Gram - cells
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41
Q

Porins in Outer Membrane:

A
  • protein channels that serve as channels for entrance/exit (not impermeable to large molecules) of small molecules
  • Can be specific or non-specific. Can be regulated
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42
Q

Functions of the outer membrane: (4)

A
  • Provides mechanical strength to cell
    -Soaks up or blocks
    access to many molecules – important for antibiotic sensitivity
  • Protects cell wall
  • Enables a substantial periplasmic space
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43
Q

What is the periplasmic space?

A
  • Space between cytoplasmic/outer membranes of Gram negative bacteria
  • Buffer between environment and cell
  • Technically, cell wall is part of periplasmic space
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44
Q

Role of periplasmic space? (4)

A

o Break down macromolecules for uptake as nutrients
o High affinity binding protein for nutrients
o Detoxify harmful compounds
o Protein folding - disulfide bond formation

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45
Q

S-layers?

A
  • Rigid/permeable monolayer of protein or glycoprotein that protect bacteria from bacteriophage or bacterial pathogens from host defences
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46
Q

Difference between capsule and slime layer?

A

Capsules are organized into a matrix and attached to the cell – slime layers
are loosely attached, less organized

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47
Q

Similarities between capsule and slime layer?

Hint: 3 similar functions

A
  1. cell adhesion
  2. protection from host immune system
  3. prevent dehydration
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48
Q

Conjugative pili?

A

transfer of genetic
material between bacterial using a pilus
bridge

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49
Q

Fimbria?

A

a pilus that mediates attachment to a surface or another cell

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50
Q

What molecules can cross freely across cytoplasmic membrane?

A

o Small uncharged, non-polar molecules

o E.g. - Dissolved O2, dissolved CO2, small alcohols/fatty acids

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51
Q

What molecules can not cross freely across cytoplasmic membrane?

A

large/charged molecules, ex: Na+/K+

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52
Q

Passive transport?

A

No energy needed for bringing molecules down concentration gradient.

-Simple diffusion, Facilitated diffusion (requires transporters)

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53
Q

Active transport?

A

need energy for uptake against concentration gradient

  • Simple transport, ABC transporters, Group transport
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54
Q

Diffusion vs Osmosis?

A

Diffusion: The net from area of high concentration to area of low concentration

Osmosis: is the diffusion of water through a selectively permeable
membrane along its concentration gradient.

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55
Q

Facilitated Diffusion:

A

Diffusion of
molecules across the membrane using
membrane protein that acts as a channel. Can be nonspecific, specific, regulated.

Ex: Porins!!

56
Q

Where does energy needed for Active Transport come from?

A

stored chemical energy (e.g. ATP hydrolysis) or from
transporting another
molecule along its concentration gradient (coupling)

57
Q

Similarity b/w Symporters and Antiporters?

A

use proton motive force to power the transport of a different molecule against its
gradient

58
Q

Difference b/w Symporters and Antiporters?

A

Symport: Both molecules travel same direction

Antiport: One molecule in, the other out

59
Q

What is the main purpose of the Sodium Proton Antiporter?

A

– pH (and Na+) homeostasis!

  • Expel Na+ from cell under high salt conditions
  • Lower pH of cell under alkaline conditions
60
Q

Lac permease symporter?

A

uptake of lactose and
disaccharides into
the cell through Proton motive force

61
Q

What is Group Translocation?

A
  • active transport
  • Transported substance is bound by a transporter and is chemically modified during transport
  • ex: Phosphorylation of sugar molecules
62
Q

What are ABC transporters?

A

-active transport
- ATP binding cassette (ABC transporters) use
ATP to power the transport of substances across
the cytoplasmic membrane

63
Q

Role of Periplasmic Binding Proteins?

A

to capture their ligand within the periplasm

64
Q

Explain Vit B12 Transport?

A
  • ABC transporter
  • OM barrel protein BtuB binds B12 with high affinity, transports across OM using energy from TonB complex (via proton motive force)
65
Q

Define Motility:

A

The ability to propel your own movement

66
Q

Flagellum?

A
  • a long, thin filament that acts like a propeller

- rotated using a motor that is anchored in the cell envelope

67
Q

Peritrichous Flagellum?

A

many across pole/body

68
Q

Monotrichous/ Polar Flagellum?

A

single at one pole

69
Q

Lophotrichous Flagellum?

A

many, all at one pole

70
Q

Amphitrichous Flagellum?

A

Both poles

71
Q

Atrichous Flagellum?

A

No flagella at all

72
Q

Direction of Peritrichous Flagellum?

A

CCW: Longer “runs” - cell moves forward

CW: Short “tumbles” – bundle falls apart – bacteria tumbles, starts new random orientation/direction

CCW –> CW: dictates direction of movement

73
Q

Reversible Flagellum vs Unidirectional Flagellum?

A

Reversible flagellum: rotation in opposite
directions reverses direction of movement

Unidirectional flagella: rotation stops/starts; Random movement during “stops” change direction of bacterium

74
Q

3 Segments of the Flagellum:

A

1) Filament: Long, thin propeller – drives movement
2) Hook: Adaptor that connects filament to the basal body
3) Basal body: Core of the structure. Powers rotation of filaments

75
Q

What drives rotation in the flagellar motor?

A

proton motive force

76
Q

Rings in the Central Rod and their roles:

A
MS ring (cytoplasmic membrane): rotates rod and 
hook and filament

P ring (peptidoglycan)/ L ring (outer membrane) :bearings (or bushings) to help rotation

C ring (cytoplasm): generating torque, switching motor direction, flagellin secretion

77
Q

What does Gram + Flagellum lack?

A

lacks P/L rings – only contains C/MS rings

78
Q

Flagellin?

A

The long filament that drives movement is made of thousands of copies of a single protein
- highly conserved in bacteria!

79
Q

Type 3 Secretion System?

A

to export flagellin: a related system is used as a protein toxin injection system by certain bacterial pathogens

80
Q

Synthesis of Flagellum?

A

MS/C RING –> STATOR –> P RING –> L RING–> EARLY HOOK –> CAP –> FILAMENT
(Made from inside —> out)

81
Q

List 2 Variations of flagellar motility:

A
  1. use Na+ gradient to drive rotation
  2. Axial filament - rotation results in corkscrew motion of entire bacterium

Note: flagellar motility is highly regulated!

82
Q

Chemotaxis:

A

Movement in the direction of gradients of increasing

or decreasing concentration to particular chemicals

83
Q

Phototaxis vs Aerotaxis:

A

Phototaxis: Movement toward/away from light

Aerotaxis: Directed motility in response to O2

84
Q

Twitching motility?

A

extend from cell –> attach onto surface –> retraction –> pull bacteria foward –> let go –> repeat

-type IV pilus

Note: like a grappling hook, would NOT be useful in liquids (aquatic bacteria)

85
Q

Inclusions?

A

bodies or aggregates within the cell

86
Q

Microcompartments?

A

protein shells than encase specific enzymes/metabolites/cofactors
that carry out specific metabolism

87
Q

Which prokaryotes store carbon as lipids and form large granules?

A
  • poly-β-hydroxyalkanoates (PHA)

- poly-βhydroxybutyric acid (PHB)

88
Q

Polyphosphate Granules?

A

excess of phosphate – broken down

to produce nucleic acids/phospholipids

89
Q

Sulfur Storage Granules?

A

oxidize reduced sulfur compounds for energy/CO2 fixation

90
Q

Gas Vesicles?

A

-Protein structures that keep water/solutes
out, but allow gas in
-buoyancy!!
-ex: cyanobacteria

91
Q

Carboxysomes?

A

concentrate enzymes involved in carbon fixation – increases efficiency and reduces unwanted side
reactions

92
Q

Other functions of Microcompartments?

A
  • protect cell against toxic/reactive intermediates/biproducts
93
Q

Endospores?

Hint: ‘protective long storage mechanisms’

A
  • highly differentiated, dormant cells that can
    survive starvation
  • Gram +
  • extremely resistant – heat, radiation, drying,
    nutrient depletion, chemicals
94
Q

Vegetative cells?

A

normal, metabolically active, growing/dividing cells

differentiate into endospores upon nutrient deprivation

95
Q

What features are shut down in endospores? (3)

A
  1. enzymatic activity
  2. respiration rate
  3. Macromolecular synthesis
96
Q

What features provide resistance/stability to endospores? (4)

A
  1. calcuim content
  2. dipicolinic acid
  3. water content
  4. small acid soluble spore proteins
97
Q

Expand on the features provided resistance/stability? Like why they important?

A

Dehydration: water is < 25%
- increases resistance to desiccation, heat, chemicals – inactivates cell’s enzymes

Dipicolinic acid (DPA) – complexed with Ca2+ 
-dehydration process,  binds/stabilizes DNA
DPA structure

Small acid soluble proteins (SASPs) -
Bind DNA, protect it from damage
(UV, heat, denaturation, mutation). Also act as carbon/energy source during germination/outgrowth

98
Q

Structure of Endospores?

A

Core is where DNA/ribosomes are housed
– will become the vegetative cell

Cortex – peptidoglycan layer

Two membranes – this “outer
membrane” – no LPS

Coat – protective protein layer comprised
of many different proteins

99
Q

Major Events of Endospore Formation:

A

Endospore -> Germination -> Vegetative Cell -> Growth/Cell Division/ DNA replication ->Forespore contained in mother cell -> Engulfment ->Both inner/outer membrane formed -> Coat/Cortex formed -> Maturation (Ca2+, SASP, dipicolinic acid) -> Mother cell lysis -> cycle repeat

100
Q

What do eukaryote cells have that’s cool and what do they do?
(its like a huge mansion compared to a cottage)

A

they have membrane bound internal structures that have their own functions creating complexity and organizations (like my mansion has a pool room and theatre)

101
Q

NUCLEUS

A

separates genetic material from rest of the cell
trnsc/tranl uncoupled-ribosomes outside nucleus
proteins related to DNA must be translocated into nucleus

102
Q

MITOCHONDRIA

A

universal among eukaryotes
energy centre of cell
own genomes, own ribosomes
evolved from alphaproteobacterium

103
Q

GOLGI AND ER

Hint: post office Golgi

A

Modify and sort
Glycosylation
molecules packaged in vesicles and then trafficked

104
Q

Know the other components of cells like cytoskeleton, vacuoles, lysosomes, chloroplasts, vesicles, cell wall,

However I feel like all of us have studied these multiple times so focus on memorizing stuff we don’t know

A

KEEP GOING WOOHOOOO

105
Q

What eukaryotic cell do we use as a model

106
Q

LECA

A

last eukaryotic common ancestor

107
Q

how many supergroups of eukarya are there ?

A

five, paanch, cinqo, cinq

108
Q

who has more metabolic diversity? prokaryotes or eukaryotes?

A

prokaryotes

109
Q

Algae

A

includes microbes and non-microbes, usually aquatic

hundreds of thousands species

110
Q

Fungi

A

includes microbes and non-microbes, closely related to animals, non-motile
CHITIN (polysaccharide cell wall defining feature)
some fungi are human or pathogens

111
Q

This fungal pathogen is the most common cause of a yeast infection

A

Candida albicans

112
Q

Yeast convert carbohydrates into

A

CO2 and alcohol via fermentation

113
Q

Amoeba (like the cute amoeba sisters)

A

single celled eukaryotic, inhabit freshwater and soil, amoebozoa is a diverse phylum of eukaryotes

114
Q

what do amoeba use for locomotion (sounds like some kind of superpower)

A

pseudopods

115
Q

Pseudopods

A

temporary projections that stick out and help move

116
Q

Eukaryotic P-words

A

protist, plankton, parasite

117
Q

protist

A

historical term used to describe eukaryotic microbe that is not a plant, animal or fungues

118
Q

plankton (what’s the krabby patty recipe?)

A

drifters…they just drift in water or air

“so we drifting…wave after wave”

119
Q

parasite

A

symbiotic relationship, some pathogens

120
Q

T/F: Archaea domain shares a more recent common ancestor with Eukarya than Bacteria

121
Q

T/F: Bacteria are commonly known as extremophiles since they tend to live in extreme environments.

A

False. It’s Archaea that are extremophiles.

122
Q

Similarities between Archaea and Bacteria?

A
  • both prok.
  • both usually single cell
  • overall similar sizes/morphologies
123
Q

Archaea cell membrane?

A
  • ether-linked
  • isoprenoid (different subunit compared to the lipid fatty acids in eukarya/bacteria)
  • lipids contain side branches and rings
124
Q

Some archaea produce __________phospholipids (a lipid monolayer) but the lipid tails are __________.

A

transmembrane; joined

joined lipid tails –> different from eukarya/bacteria

125
Q

T/F: S-layers are present in both archaea and bacteria

A

True! BUT, they have different functions in each (covered in a diff. flashcard).

126
Q

For majority of archaea, S-layers act as the ___________.

127
Q

In bacteria, S-layers act as ____________ structures.

A

supplementary

128
Q

Archaea Cell Walls

A
  • made of simple polymer: pseudomurein
  • doesn’t have D-amino acids, different sugar linkages
  • NAM replaced with N-acetyltalosainuronic acid
129
Q

What is a hamus? (hami for plural)

hint: ‘grappling hook’ in archaea

A

in archaea, fixes cells to a surface to mediate biofilm formation

130
Q

The archaellum

you guessed it: archaea + flagellum

A
  • its like a flagellum BUT evolved separately from bacteria
  • chemotactic
  • driven by ATP hydrolysis (DIFFERENT from bacteria!!)
  • built from inside out - Type IV pilus
131
Q

T/F: Generally, archaea swim slower than bacteria.

132
Q

An unusual archaea example: halophile with a long name

hint: i’m salty about how long this name is….

A

Haloquadratum walsbyi

  • halophile –> lives in saturated salt lakes
  • unique morphology: thin squares morphology ( like shreddies crackers)
  • high Surface to Volume ratio
133
Q

Asgard Archaea example

hint: add ‘archaeota’ at the end of an amazing marvel character’s name

A

LOKIarchaeota

- there’s also thorarchaeota, odinarchaeota etc. but not as important to know

134
Q

Asgard Archaea Discoveries

A
  • discovery bridged the gap between archaea and eukarya (get it…‘bridge’ like the Bifrost - k i’ll stop)
  • in 2017, many asgard archaea discovered with new ‘eukaryotic functions’
  • culturing asgard archaea finally happening in 2020 - after 12 years of efforts!
135
Q

Asgard Archaea general facts

A
  • Lokiarchaeota was found first
  • found in anaerobic marine sediments
  • contain eukaryotic - specific proteins (ex. vesicle trafficking)