topic 19c: chromatography

Question 1

what does chromatograph do

Answer 1

separates components of a mixture between a mobile phase and a stationary phase

Question 1

gas chromatography: mobile and stationary

Answer 1

mobile: an inert gas
stationary: a liquid on an inert solid

Question 2

HPCL mobile and stationary

Answer 2

mobile: liquid
stationary: solid eg silica

Question 3

a solid stationary phase separates by …

Answer 3

adsorption

Question 4

a liquid stationary phase separates by

Answer 4

solubility

Question 5

2 types of column chromatography

Answer 5

GAS chromatography
high performance liquid chromatography

Question 6

GC used to

Answer 6

separate mixtures of VOLATILE liquids

Question 7

how does column chromatography separate substances

Answer 7

based off different retention times in the column

Question 8

what tp use to visualide TLC

Answer 8

UV light

Question 9

when might u use chromatography

Answer 9

• forensices
  drug testing in sport

Question 10

longer retention time means…

Answer 10

!!!!greater attraction to stationary phase!!!
weaker attraction to mobile phase

Question 11

amino acids and polar stationary phase

Answer 11

think of ZWITTERIONS and charge

Question 12

why might you be missing a spot ?

Answer 12

• same rf value as another
• same pigment present twice

Question 13

TLC: diffeent rf values

Answer 13

• different solubiltiy in the MOBILE PHASE
• different ADSORPTION to the stationary phase

Question 14

TLC mobile and staionary

Answer 14

mobile: liquid solvent
stationary: SOLID so ADSORPTION