Topic 10 - Prokaryotic Regulation

Question 1

Encode products the affect expression of other genes

Answer 1

Regulatory Genes

Question 2

Encode a protein product that effects the structure of the cell.

Answer 2

Structural Genes

Question 3

Any sequence of DNA that is not converted into other form but functions as it is in situ, affecting only the DNA to which it is physically linked.

Answer 3

Cis-acting

Question 4

The product of a DNA, which may be a protein or an RNA, that diffuses away from its site of synthesis to work elsewhere.

Answer 4

Trans-acting

Question 5

Structural genes with related functions that are grouped together.

Answer 5

Polycistronic mRNA

Question 6

Prokaryotic DNA that clusters genes with similar functions.

Answer 6

Operons

Question 7

When the binding of the regulatory protein to the operator turns transcription on.

Answer 7

Positive, Activator

Question 8

When the binding of the regulatory protein to the operator turns transcription off.

Answer 8

Negative, Repressor

Question 9

When the presence of a signal turns transcription on.

Answer 9

Inducible

Question 10

When the presence of a signal turns transcription off.

Answer 10

Repressible

Question 11

The regulatory gene in the lac operon in E. Coli

Answer 11

lacI

Question 12

Structural gene that encodes for beta-galactose, which is used in the process of turning lactose into glucose.

Answer 12

lacZ

Question 13

Structural gene that encodes for permease which allows lactose into the cell.

Answer 13

lacY

Question 14

Regulatory gene, Operator in the lac operon.

Answer 14

lacO

Question 15

Lactose regulation of lac operon

Answer 15

Negative Inducible

Question 16

Secondary messenger for glucose availability, when glucose is high, this is low and vise versa.

Answer 16

cAMP

Question 17

Regulatory protein that is activated when cAMP binds to it, resulting in transcription.

Answer 17

Catabolite Activator Protein (CAP)

Question 18

Glucose regulation of lac operon

Answer 18

Positive Inducible

Question 19

Tryptophan regulation of trp operon

Answer 19

Negative Repressible

Question 20

Finetuning, when transcription and translation begin, but are prematurely terminated by a signal.

Answer 20

Attenuation

Question 21

Regulation of transcription and translation of the trp operon based on the presence of trp charged tRNA

Answer 21

trp Attenuation

Question 22

RNA forms a secondary structure and the antiterminator allows RNA polymerase to continue in the 1-2 region.

Answer 22

Low trp

Question 23

Terminator sequence forms and stops RNA polymerase in the 3-4 region.

Answer 23

High trp

Question 24

A transformation of bacteria used to express a protein.

Answer 24

Expression Vectors