Thin Layer Flashcards
The SP consists of a….
Thin layer of small particles attached to a flat plate - The TLC plate.
Process is carried out on a….
Plate
Stationary phase is…
A silica gel
Same as paper chromatography, but uses a….
Plate instead of paper.
Sample is placed on a plate at starting point, the solvent MP slowly….
Rises up the silica gel.
The compounds move up the plate at…..
Different rates and the mixture separates.
The solvent front is…
Marked about 3/4 of the way up the plate.
The distance from the start point for each solvent can be measured and from this a
Retardation factor can be calculated.
Equation for retardation factor (Rf)
Rf = distance of spot from origin / distant of solvent from origin.
The Rf value relates to a specific compound and a ….
Specific eluant and SP.
Process is easy to use for…..
Coloured compounds.
Can use the Rf factor to identify unknown substances by….
Comparing it with Rf values of known substances.
TLC can be use quantitively to…..
Diagnose syphillis.
TLC can be used qualitatively to….
Detect illegal drugs.
TLC is a quick technique and can be used to check….
When a reaction has gone to completion.
