Bonded-Phase Chromatography

Question 0

Dependent on chemical properties of the….

Answer 0

Compounds in the mixture.

Question 1

Liquids are chemically bonded to….

Answer 1

Solid support, such as silica.

Question 2

Mobile phase can be a….

Answer 2

Liquid or a gas.

Question 3

Normal-Phase chromatography is when the SP is…..

Answer 3

Polar and the mobile phase is non polar.

Question 4

In normal phase chrom, the non polar molecules…..

Answer 4

Remain in the MP and are eluted earlier than the polar compounds, which are attracted to the more polar SP.

Question 5

In Reverse phase chromatography, the SP is…..

Answer 5

Non polar and the MP is polar.

Question 6

Reverse phase chromatography is more commonly used than….

Answer 6

Normal phase chromatography.

Question 7

What is a gradient elution?

Answer 7

A continuously varying proportion of solvents used to elute compounds with differing chemical properties. For example a change in polarity.

Question 8

How is a gradient elution used in reverse phase chromatography?

Answer 8

The SP is non polar. A polar MP elutes polar compounds first. Then an increasing amount of non polar solvent is added until the non polar compounds elute.

Question 9

Like attracts like, therefore….

Answer 9

Polar attracts polar

Question 10

Molecules with differing polarity repel or show a much….

Answer 10

Weaker attraction.