Gas Chromatography Flashcards
It is a type of…..
Analytical and column chromatography
Separates complex mixtures with a….
Very high resolution.
The mobile phase is a gas, such as….
Helium, an inert gas that won’t react with the compound.
Is the SP is a liquid, it is called GLC, this is common because…..
Of it’s speed and efficiancy.
Three uses of GC…
Pesticide analysis.
Analyse paints.
Identify small organic water components.
The SP is either a packed column or a …..
Capillary column.
A packed column can be upto….
10 m in length
A capillary column can be up to….
100 m in length. These are more efficient than packed columns.
Sample should be a gas or a….
Volatile liquid, which will evaporate and is stable at low temps.
How can non volatile compounds, such as nandrolone, be analysed?
The compound of interest can be derivatised to another compound to produce a substance volatile enough to analyse.
The MP carries the sample down the column, it needs to be unreactive so that it doesn’t…..
Interfere with the detector. Eg helium.
The MP flow rate is controlled by a…
Valve and should be kept at a constant pressure of 10-50 PSI.
The GC injector port may be heated to…..
Vaporise the sample.
GC systems can split the sample automatically so that….
Only a fraction of the sample goes into the column.
Separation is achieved by a temperature gradient, the most…..
Volatile compounds are eluted first.
