HPLC Flashcards
HPLC is a type of
Analytical chrom
Can use chromatography based on chemical and physical properties, including….
Size exclusion
Reverse phase chrom
Ion exchange chrom
Can be used to analyse classes of compounds, including… (3)
Salts.
Ions and proteins.
Thermally unstable compounds, eg enzymes.
Separates complex mixtures using…
Columns that can withstand high pressure and are resistant to a range of chemicals. Eg steel columns.
The columns are packed with
Small particles.
The MP is pumped through at a ….
Reasonable speed, thus improving efficiancy.
Solvents should be degassed to prevent…
Bubbles from forming. These can interfere with valve operation and also cause peaks on the detector.
Can be used for water samples to…
Identify presence of small organic contaminants.
HPLC columns have an internal diameter of
3 mm to 10 mm
Columns contain spheres of…
High purity silica, packed into a dense matrix.
Van D eq: a reduced plate height, values of A and C, leads to a…
Better separation efficiancy.
Van D eq: the B value is…
Small in HPLC
Sample loops adds a known volume of sample into the column through an….
Injector port, this is isolated from the pump and column, until moved to be connected to the column.
When the MP is pumped, the sample is…
Loaded into the column.
Silica is a good packing material because…
It has excellent absorbent properties.