THICK AND THIN BLOOD SMEAR

Question 1

these are essential diagnostic tools for malaria

Answer 1

thick and thin blood smears

Question 2

True or False. Malaria is endemic in the Philippines

Answer 2

True

Question 3

State cities in the PH where Malaria is endemic

Answer 3

Palawan
Sultan Kudarat
Davao del norte
Maguindanao
Sulu
Mindoro occidental
Tawi-tawi
Cagayan Valley
Davao City

Question 4

this involves applying a LARGER drop of blood to a slide, allowing it to dry without spreading thinly

Answer 4

thick blood smear

Question 5

this is prepared by spreading a small drop of blood into a thin layer on a slide

Answer 5

thin blood smear

Question 6

this is used to SCREEN presence of malarial parasite

Answer 6

THICK smear

Question 7

thin smears allows what?

Answer 7

malarial spp identification
quantification of parasitemia
recognition of different Plasmodium stages

Question 8

how many drops is needed in thick smear

Answer 8

3 drops or 6uL

0.006 mL

Question 9

how many drops is needed in thin smear

Answer 9

1 drop (2ul)

Question 10

Staining process of thick smear

Answer 10

Lysed or dehemoglobinized RBC

Question 11

Staining process of thin smear

Answer 11

Fixed RBCs in methanol

Question 12

Blood collection methods of thick and thin blood smear

Answer 12

capillary puncture
venipuncture with tube containing anticoagulant

Question 13

specimen of venipuncture with tube containing anticoagulant

Answer 13

whole blood

Question 14

specimen of capillary puncture

Answer 14

capillary blood

Question 15

where is capillary blood usually taken from adults and infants

Answer 15

Adults = middle and ring fingers
Infants and small children = heel

earlobe was formerly used as well

Question 16

Angle used in blood smear

Answer 16

~ 45º

Question 17

state the common errors in preparing blood films

Answer 17

drop of blood too large or too small

chipped or dirty edge of spreader

slide contaminated with fat or grease

Failure to keep entire edge of the spreader slide against the slide while making the smear

Question 18

What type of stain is Giemsa stain?

Answer 18

Romanowsky based stain

Question 19

What are the two main components of Giemsa stain?

Answer 19

eosin
methylene blue

Question 20

this stains the parasite nucleus RED

Answer 20

eosin

Question 21

this stains the cytoplasm BLUE

Answer 21

methylene blue

Question 22

does thick smear used a fixative

Answer 22

no

Question 23

What is used as a fixative for a thin blood smear?

Answer 23

methanol

Question 24

What is used to dehemoglobinize a thick blood smear?

Answer 24

acetic acid

Question 25

What concentrations of Giemsa working solution are commonly used?

Answer 25

10% or 3% Giemsa solution

Question 26

What are the two methods of fixation in Giemsa staining?

Answer 26

**rapid method** - dropping methanol onto the thin film **slow method** - dipping the slide in methanol for 2 seconds

Question 27

Why is methanol used for fixation?

Answer 27

preserves cell morphology by fixing the blood smear to the slide.

Question 28

How long should slides be stained using the slow method?

Answer 28

45-60 minutes.

Question 28

How long should slides be stained using the RAPID method?

Answer 28

8-10 minutes

Question 29

Why should stain not be poured directly off the slide?

Answer 29

the metallic green surface scum can stick to the film and interfere with microscopy.

Question 29

where is Giemsa stain discarded

Answer 29

chemical waste container labeled for organic solvents.