FILARIAE Flashcards
state filarial spp which are pathogenic
Wuchereria bancrofti
Brugia malayi
Loa loa
Onchocerca volvulus
state filarial spp which are nonpathogenic
Mansonella ozzardi
Mansonella perstans
What are the two known morphologic forms of filarial spp
Adult worms
Microfilariae (larvae)
where are falarial spp mostly seen
within blood or tissues
Filarial spp are under the phylum?
Nemathelminthes
Filarial spp are under the class?
Nematoda
creamy white and threadlike appearance
adult worms
slender and size ranges under 150 um to 350 um in length
microfilariae
state the two key characteristics helpful in speciating the microfilariae forms
distribution of nuclei
presence or absence of sheath
True or False. The Life Cycle is same for all members of the filariae
True
How many infective larvae/microfilariae is required to initiate human infection
1 to 4
resulting adult worms reside in
lymphatics
subcutaneous tissue
intestinal body cavities
where to live microfilariae take up residence
blood or dermis
who lays live microfilariae
fertilized adult FEMALE worms
is there periodicity in filarial spp
Yes
a pheno menon whereby the parasites are present in the bloodstream during a specific time
periodicity
parasite is present in the blood during the NIGHT
nocturnal
parasite is present in the blood during the day
diurnal
whole blood sample may also be collected via
knott technique
membrane filtration
timing of the occurences is not clear-cut
Subperiodic
primary method of diagnosis of filarial spp
microscopic examiniation of the microfilariae of either from:
giemsa stained blood
tissue scrapping of an infected nodule
composition of knott technique
1 ml of blood mixed with 140 ml of 2% formalin
state the process of the knott technique
1 ml of blood mixed with 140 ml of 2% formalin; centrifuged; supernatant is discarded; sediment is studied and spread like a thin blood film; fixed and stained and is then view under the microscope
this makes use of syringe attached to a Swinney filter holder
membrane filtration
membrane filtration composition
1 ml of fresh/anticoagulated blood (in syringe) & lyzed by adding 10ml distilled water > lyzed blood passes through the Swinney membrane filter > microfilariae is recovered
true or false. membrane filter can be examined like a wet smear prep, fixed, dried, stained
true
microfilariae in blood
microfilaremia
concern regarding serologic tests
specificity (they are not specific)
common name for Wuchereria bancrofti
Bancroft’s Filaria
common associated disease for Wuchereria bancrofti
Bancroft’s Filariasis or Elephantiasis
More sensitive method for W. bancroft microfilariae recovery
heparinized blood
blood filtered nucleopore filter filtered content are stained & examined
peak hours for collecting the specimen infected w/ W. bancrofti
9pm to 4 am (nocturnal)
serological tests used in W. bancrofti
polymerase chain reaction assays
antigen & antibody detection
this may occur in asymptomatic patients of W. bancrofti
eosinophilia
this may occur in on the death of W. bancrofti adult worms
calcification or formation of abscesses
bancroftian filariasis results to
formation of granulomatous lesions
lymphangitis
lymphadenopathy
common name for Brugia malayi
Malayan Filaria
Common associated disease for Brugia malayi
Malayan filariasis or Elephantiasis
Common associated disease for Loa loa
Loiasis
