The Prokaryotes: Flashcards

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1
Q

About how many years ago did the first prokaryotic cells evolve, according to the fossil record?

A

Earliest prokaryotic fossils about 3.5 BYA.

In the Precambrian Era.

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2
Q

What is the basic idea behind the Oparin-Haldane hypothesis? (1938)

A

That life arose through abiogenic molecular Evolution

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3
Q

In what ways were Oparin and Haldane’s ideas similar?

A

-That early Earth was very inhospitable, due to its primitive atmosphere, with intense heat, also agreed sunlight provided the energy with its intense UV.

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4
Q

In what ways were Oparin and Haldane’s ideas different?

A
  • Oparin: atmosphere lots of methane,, water vapor, ammonia and hydrogen gas; intense heat of volcanoes, lightning and sunlight provide the energy; (the formation of coaverates)
  • Haldane: atmosphere had water vapor, carbon dioxide, and ammonia; Intense UV from sun since no ozone layer, which provided the energy; (the formation of organic molecules, in a “hot dilute soup,” covering Earth [premordial soup]
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5
Q

Stanley Miller’s 1953 Experiment were designed to test what hypothesis?

A

his experiments were designed to test Oparin-Haldane Hypothesis, using conditions of early Earth suggested by Oparin

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6
Q

What conclusions did Miller draw from the results of the experiments (1953)?

A
  • Concluded that 15% of Carbon incorporated into 4 kinds of amino acids (which builds proteins), Urea, and fatty acids (which build lipids) [The 15 % is coming from the methane (CH4)]
  • Also concluded, the experiment doesn’t work when the atmosphere contains O2, implying early Earth had no oxygen in it (it came later).
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7
Q

what was the key difference between Fox’s “Protobionts/Protocells” and actual living cells?

A

“Protocells” are like cells, however they can’t reproduce and/or replicate itself/themselves.

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8
Q

Why would it be more likely that nucleic acids evolved before enzymes evolved in living systems? Explain why scientists think that is true:

A

It is more likely that nucleic acids evolved before enzymes because nucleic acids are easier to build

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9
Q

Which type of Nucleic Acid probably evolved first: RNA or DNA? why do scientists think this is true?

A

We believe RNA came first, since its easier to build, because you only need 1 strand of nucleotides (which its also a template on how to build a protein)

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10
Q

What is the geographic range of bacteria?

A

Bacteria are ubiquitous meaning they are found everywhere, all over the place

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11
Q

3 Basic shapes of bacteria cells?

A

1-Coccus (plural=cocci)—->Round
2-Bacillius (plural=bacilli)—-> hot dog/rod shaped
3-Spirillium (plural=spirillium)–> spiral (corkscrew)

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12
Q

What are Bacteria’s most common Arrangements?

A
  • 1–Strepto–Meaning “chains”/ Chained together
  • 2–Staphylo–Meaning “grape-like clusters
  • 3–Diplo–Meaning “pairs”
  • 4–Sarcina–Cuboidal (packets of 4, 8)
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13
Q

The method of reproduction for bacteria:

A
  • No Mitosis and No Meiosis

- They do a process to replicate itself called Binary Fission (all have same DNA)

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14
Q

What are the Principle differences between Prokaryotic cells and Eukaryotic cells?

A
  • Prokaryotes do not divide by mitosis, instead through binary fission.
  • The organization of the genetic material differs, since the DNA of prokaryotic cell is not organized within a membrane enclosed nucleus, they have smaller DNA molecules called Plasmids, and DNA is often circular.
  • prokaryotes have none of the membrane-enclosed cytoplasmic organelles—(mitochondria, Golgi apparatus, and others, that are found in most Eukaryotes)
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15
Q

What are the 2 Domains of prokaryotic cells? Which domain is more closely related to Eukaryotes?

A
  • 2 Domains: Bacteria and Archaea

- Eukaryotes share a more recent common ancestor with Archaea, than they do with bacteria.

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16
Q

What roles (other than “disease-causing”) do bacteria play in the human environment?

A
  • Environmental applications: composting, bio-leaching of minerals, bio-remediation, sanitary landfill, sewage treatment.
  • Food Production: Yogurt, cheese, Intestinal bacteria (e. coli), normal skin flora
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17
Q

The size of bacteria:

A

They’re really small:

-requires staining and really good (light) microscope lenses to see them.

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18
Q

The best way of telling/classifying bacteria is?

A

by looking at the cell wall

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19
Q

Stromatolites:

A
  • Found in water or where water was
  • They form when biofilms (matted together prokaryotes) collect particles over time creating rocks, trapping the bacteria from that biofilm
20
Q

Cyanobacteria:

A
  • Photosynthetic Bacteria

- Modern day bacteria similar to stromatolites

21
Q

All bacteria cells have:

A
  • Cytoplasm
  • Ribosomes (smaller than Eukaryotes)
  • Nucleiod (DNA one big circular piece)
  • Plasma membrane
  • Cell wall (NOT selectively permeable) [gram positive/negative]
22
Q

Physical factors in the environment can affect the rate of reproduction of bacteria which include:

A
  • Temperature
  • Atmospheric Oxygen Level
  • pH levels
  • Electromagnetic radiation
23
Q

How does temperature affect the rate of reproduction of bacteria?

A
  • Psychrophile: “likes cold”
    • -5 to 20 C
    • Refrigeration, freezing doesn’t kill bacteria
  • Mesophile:
    • 20-45 C (Can be found inside us)
    • Includes plant, animal pathogens (most of what we encounter falls here)
  • Thermophile:
    • 45C and up
    • Overheating usually kills bacteria, but NOT THESE! (ex:geysers, deep sea vents)
24
Q

How does Atmospheric Oxygen Level affect the rate of reproduction of bacteria?

A
  • Aerobe: need oxygen for respiration (we fall into this) [36 ATP]
  • Anaerobe: Killed in the prescence of oxygen; Fermentation produces ATP (making 2 ATP)
  • Facultative: Can make ATP by respiration or fermentation (alcohol–>bacteria—>Acid—>Fungi)
  • Microaerophile: “likes a little but of air”; Prefers low [O2], higher [CO2]
25
Q

How does pH Levels affect the rate of reproduction of bacteria?

A
  • Acidic: (ex; stomach ulcers)
  • Neutral: (most bacteria prefer this)
  • Alkaline: (ex: soil)
26
Q

How does Electromagnetic Radiation affect the rate of reproduction of bacteria?

A
  • Gamma Radiation, X-rays: Higher Penetrability; Ionizing radiation (causes formation of ions) free radicals.; Extensive cellular damage; making dark places best for bacteria!!!!
  • UV radiation: Lower Penetrability —->surface sterilization; thymine dimers (causing thymine to fuse)
27
Q

The only things that kills bacteria are:

A
  • UV radiation
  • Gamma Radiation
  • X-Rays
28
Q

Modes of nutrition for bacteria vary widely; what is Bacteria’s metabolism:

A
  • Autotrophs
  • Heterotrophs:
  • Decomposers:
29
Q

Autotrophs;

A

Makes food for themselves

30
Q

Heterotrophs:

A

Gets food from an outside source

Relies on some other means of a food source

31
Q

Decomposers:

A
  • Special case of heterotrophs
  • Breaks down dead stuff/gets its food source from it
  • (digests nutrients in the compost)
32
Q

Bacteria Motility: (How do bacteria get from Point A to Point B?)

A
  • Flagella
  • Axial Filaments (aka “endoflagella”)
  • Gas Vesicles;
33
Q

Flagella in Bacteria Motility:

A

In bacteria cells it rotates (instead of whipping itself further like a sperm)

34
Q

Axial Filaments (aka “Endoflagella”) in Bacteria Motility:

A

On the inside, when rotation happens it looks like a drilling motion

35
Q

Gas vesicles in Bacteria Motility:

A
  • Found in cyanobacteria

- Can cause bacteria to float or sink by absorbing/releasing

36
Q

Bacteria and its adaptations to the environment:

A
  • Antibiotic Resistance:
  • Endospore Formation
  • Extracellular Secretions `
37
Q

Antibiotic Resistance and Bacteria:

A

The bacteria can develop a resistance towards a drug, With the discovery of Penicillin it helped with this!
-Can be tested in a lab to find if it is Susceptible or Resistant

38
Q

Discovery of Penicillin:

A

In 1928, by Alexander Fleming

  • (Where mold/fungus was found, there was no bacterium—on dirty lab plates he observed it)
  • Cures gonorrhea
39
Q

Endospore Formation and Bacteria:

A
  • (not in all bacteria)
  • endospore: Metabolically inactive dormant structure produced by some bacteria species
  • Can be detected with special staining techniques
  • bacteria makes endospores when environmental conditions aren’t good.
40
Q

Extracellular secretions and Bacteria:

A
  • (slime layers, capsules, biofilms)
  • [Making bacteria:]
    • Mainly amino acids and sugars (very sticky)
    • Protection from immune system chemicals (capsules)
    • Sticky (to themselves, surfaces, host tissues
41
Q

What is bioremediation?

A

ex: Cleaning up an oil spill from a beach using bacteria which eats oils.

42
Q

What indicates Archaea to be different than other domains?

A

its rRNA sequence indicates differences between Archaea and other domains.

43
Q

Name two major differences between Archaea and Bacteria

A

Archaea have no:

  • peptidoglycan in cell walls
  • Distinctive lipids in plasma membranes of some.
44
Q

Name the four major extremeophiles:

A
  • Extreme halophiles (likes salt)
  • Extreme thermophiles (likes heat)
  • Acidophillic (likes acidic conditions)
  • Alkallphilic (likes basic/metal conditions)
45
Q

Methanogens reduce CO2; how much methane is produced annually?

A

2 billion tons of methane is produce a year.