The neurone Flashcards
How can it be established that Cl- is distributed passively across neuronal membranes?
Their Nernst potential (Ecl) is approximately equal to the resting membrane potential (Vm).
What is the Donnan product rule?
[K+]out[Cl-]out = [K+]in[Cl-]in
Why do developing neurones have large numbers NKCC1 channels?
- Allows for active transport of Cl- ions into the neurones, which increases [Cl-].
- This allows for spontaneous efflux of Cl- out of neurones at rest as a result of opened Cl- channels, causing spontaneous depolarisation.
- Spontaneous depolarisation of developing neurones generate APs that are important for mediating development.
What are the different components of the NT exocytosis mechanism?
- Ca2+ sensor: Synaptotagmin
- V-SNARE: Synaptobrevin
- T-SNARE: Syntaxin & SNAP-25
What is the relationship between post-synaptic depolarisation (V) and pre-synaptic [Ca2+]?
V is proportional to [Ca2+]4
What are the criteria needed for a chemical to be regared a NT?
- It must be present in pre-synpatic terminal.
- It must be released into synaptic cleft from pre-synaptic terminal on stimulation.
- Added chemical must have same post-synaptic effect as released NT.
- Action of added transmitter must be inhibited by same inhibitors as natural transmitter.
What are the classes of NTs present in the CNS?
- Amino acids
- Bioactive amines
- Purines
- Neuropeptides
- Gaseous NTs
What are the amino acid transmitters?
- Glutamate - Excitatory
- GABA (γ-amino butyric acid) - Inhibitory
- Glycine - Inhibitory
What are the bioactive amines?
- Catecholamines
- Dopamine
- Serotonin
- Histamine
- Acetylcholine
What are the purines?
- ATP
- Adenosine
What are the gaseous transmitters?
- NO
- CO
- H2S
What is the significance of gaseous transmitters?
Unlike the other classes of NTs, they are not pre-made and stored in the pre-synaptic membrane as they are highly diffusible. Instead, they are synthesised on demand.
What are the ionotropic glutamate receptors?
- NMDA
- Non-NMDA:
1. AMPA
2. Kainate
What are the approximate timescales of action for different NT receptors?
- Ionotropic: msec
- Metabotrophic:
1. GPCRs: sec - min
2. RTKs (hormones & enzymes): Min - days
What are the 2 categories of receptor types?
- Long receptor: Sends axons straight to the CNS from area of reception.
- Short receptors: Synapses with second order cell that sends axons into the CNS.
What is the sequence of events in long receptor signal transduction?
- Stimulus causes ion channels to open.
- Receptor potential proportional to stimulus magnitude created.
- RP with sufficient magnitude triggers APs.
- Frequency of APs proportional to magnitude of RP.
What are examples of long receptors?
- Somatosensory receptors
- Olfactory receptors
What is the sequence of events in short receptor transduction?
- Stimulus opens ion channels in receptor cell.
- RP proportional to magnitude of stimulus generated.
- RP conducted passively along receptor cell until it reaches pre-synaptic membrane, where it causes voltage-gated Ca2+ channels to open.
- Influx of Ca2+ results in induction of exocytosis of NT vesicles and release of NTs into synaptic cleft.
- NT binds to post-synaptic receptors and triggers EPSP.