The Microscope

Question 1

What two structures are used to focus on the specimen?

Answer 1

Fine adjustment and coarse adjustment knob

Question 2

Why do we never use the coarse adjustment knob on high power?

Answer 2

It can crack your slide

Question 3

What happens if you use direct sunlight to illuminate your specimen?

Answer 3

You can damage your eyes

Question 4

What are the objective lenses?

Answer 4

Low, medium and high power

Question 5

What is the power of the low power objective lens?

Answer 5

4x

Question 6

What is the power of the medium power lens?

Answer 6

10x

Question 7

What is the power of the high power lens?

Answer 7

40x

Question 8

What is the diameter of the field of view of low power?

Answer 8

4 mm

Question 9

What is the diameter of the field of view under medium power?

Answer 9

1.5mm

Question 10

What is the diameter of the field of view under high power?

Answer 10

0.4mm

Question 11

How should you carry the microscope?

Answer 11

One hand gripping the arm, the other hand supporting the base

Question 12

What are dry mounts for?

Answer 12

For objects that are not alive

Question 13

What are the steps of a dry mount?

Answer 13

• place specimen on a dry slide

- cover with a cover slip

Question 14

What are wet mounts for?

Answer 14

They permet the object to be better seen

Question 15

What are the steps for a wet mount?

Answer 15

• place specimen on the slide
• with eyedropper, Place one drop of water on the specimen
• place cover slip at a 45° angle and let go
• blot excess water with a pencil eraser

Question 16

How do you find the total magnification?

Answer 16

Ocular lens x objective lens magnification

Question 17

How many micrometers are in one millimetre?

Answer 17

1000 micrometers in one millimetre

Question 18

How many millimetres in 1000 micrometers?

Answer 18

1 millimetre in 1000 micrometers

Question 19

How do you find the size of something under the microscope?

Answer 19

Diameter of the field of view
—————————————
Number of objects that fit across the diameter of field of view

Question 20

What happened to the diameter of the field of you as you move from low to high magnification?

Answer 20

The diameter of the field of view gets smaller

Question 21

Explain why the size of objects viewed under high-power is usually recorded in micrometres?

Answer 21

Millimetres are too large to measure the field of view. You need a smaller measurement which are micrometres

Question 22

Which magnification would be the best for looking at several objects at the same time?

Answer 22

The low-power objective lens because it has the lowest magnification and the largest field of view so you can see all objects at the same time