The cell cycle

Question 1

DNA replication

Answer 1

1) DNA helicase breaks the hydrogen bonds between the complementary base pairs between the two strands in a double helix, double helix unwinds

2) Each of the seperated parentsl DNA strands acts as a template, free floating nucleotides within the nucleus are attached to their complementary base pairs

3) The adjacent nucleotides are joined together to form a phosphodiester bond by a condensation reaction. DNA polymerse catalyses this to make a new strand

Question 2

Describe the role of DNA polymerase in the semi conservative replication of DNA

Answer 2

connects activated free nucleotides together

to form phosphodiester bonds in a condensation reaction

Question 3

Suggest two environmental variables which could be changed to increase h growth rate of cells

Answer 3

Increased conc of glucose —-> increased resp

Increased temp —> increased enzyme activity