TESTS FOR DETOXIFICATION FUNCTION Flashcards
It is used to assess the extent of liver damage.
ENZYME TESTS
ENZYME TESTS
located intracellularly which is inside the cell
ENZYME
ENZYME TESTS
enzyme in hepatocyte is liberated’ goes to
circulation
Damage in the liver or cell of the liver
Indicator of cell injury in early or localized liver
disease (hepatocellular/functional disease)
ENZYME TESTS
Hepatic enzymes: to assess liver damge
o ALT
o ?
o ?
o ?
o ?
o ?
o ALT
o AST
o “5 NT” 5 PRIME NUCLEOTIDASE
o “GGT”GAMMA GLUTAMAE
o LDH
o OCT
Produce from deamination of amino acids
AMMONIA
AMMONIA
Ammonia should be converted to urea through _____________ which occur in the liver.
KREBS HENSELEIT CYCLE
AMMONIA
Diagnostic marker of hepatic failure = PROBLEM
IN THE LIVER = ?
UREA WILL NOT HAPPEN AND
UREA WILL NOT FORMED!
AMMONIA
INCREASE IN AMMONIA
neurotoxin
AMMONIA
it will converted to glutamate which will cause coma
AMONIA NOT CONVERTED TO UREA
AMMONIA
Detoxified into urea (liver), then eliminated by
the ?
kidneys (urine)
AMMONIA
Reference value: ?
19-60 ug/ dL
AMMONIA
METHODOLOGY
Preferred specimen?
Venous blood
AMMONIA
Plasma can be used using?
o Heparin, EDTA, Potassium oxalate
o Kept in ice water during transport
AMMONIA
Specimen precautions:
Smoking:
can falsely elevate ammonia
by 100-200 ug/L per cigar; not only
patient the phlebotomist should not be
exposed to cigar
