Normal SPE Pattern

Question 1

1st band most abundant – fastest
and most anodal

Reference value: 3.5- 2.5.- g/dL (53-65%)

Answer 1

Albumin band

Question 2

2nd most – alpha 1 antitrypsin (AAT)
90%

Reference value: 0.1-0.3 g/dl (2.5-5%)

Answer 2

α1 Globulin band

Question 3

Reference value: 0.6- 1.0 g/dL (7-13%)

Answer 3

α2 Globulin band

Question 4

Reference value: 0.7- 1.1 g/dL (8-14%)

Answer 4

β Globulin band

Question 5

• immunoglobulins

Reference value: 0.8-1.6 g/dL (!2-22%)

Answer 5

γ Globulin band

Question 6

Part of the total protein which is most abundant
among protein

Answer 6

ALBUMIN

Question 7

ALBUMIN

synthesize 9-12 grams/day albumin

Answer 7

Liver

Question 8

Concentration is directly proportional to the
severity of ______

Answer 8

hepatic disease

Question 9

Globulins are precipitated; tinatanggal yung globulin

Answer 9

SALT PRECIPITATION ♥

Question 10

Albumin is quantitated using biuret reaction

Answer 10

SALT PRECIPITATION

Question 11

the protein is charged positively

Answer 11

DYE BINDING

Question 12

most commonly used;
sensitive dye for albumin

Answer 12

Bromcresol Green (BCG)

Question 13

most specific dye

Answer 13

Bromcresol purple (BCP)

Question 14

can be used but not specific for
albumin

Answer 14

Methyl Orange

Question 15

more specific to albumin than methyl orange

Answer 15

2,4’-hydroxyazobenzene-benzoic acid (HABA

Question 16

is negatively charge which is bind
to positive (albumin)

Answer 16

Amniotic dye

Question 17

Validate if globulin is higher than albumin

Answer 17

ALBUMIN/GLOBULIN RATIO

Question 18

globulin is higher than
albumin

seen in cirrhosis, multiple myeloma and
Waldenstroms magcroglobolinemia

Answer 18

Inverted A/G ratio

Question 19

PROTHROMBIN TEST

prior to testing
administered vit K intramuscularly 10mg for 1-3
days.

Answer 19

Vitamin K Response Test

Question 20

PROTHROMBIN TEST

indication that there is a problem in liver
synthetic; HEPATIC

Answer 20

If PROTHROMBIN time is prolonged

Question 21

PROTHROMBIN TEST

prothrombin time is normal the problem
is EXTRAHEPATIC

Answer 21

After administration of VIT K

Question 22

It differentiates intrahepatic disorder from
extrahepatic disorder

Answer 22

PROTHROMBIN TEST