Normal SPE Pattern Flashcards
1st band most abundant – fastest
and most anodal
Reference value: 3.5- 2.5.- g/dL (53-65%)
Albumin band
2nd most – alpha 1 antitrypsin (AAT)
90%
Reference value: 0.1-0.3 g/dl (2.5-5%)
α1 Globulin band
Reference value: 0.6- 1.0 g/dL (7-13%)
α2 Globulin band
Reference value: 0.7- 1.1 g/dL (8-14%)
β Globulin band
- immunoglobulins
Reference value: 0.8-1.6 g/dL (!2-22%)
γ Globulin band
Part of the total protein which is most abundant
among protein
ALBUMIN
ALBUMIN
synthesize 9-12 grams/day albumin
Liver
Concentration is directly proportional to the
severity of ______
hepatic disease
Globulins are precipitated; tinatanggal yung globulin
SALT PRECIPITATION ♥
Albumin is quantitated using biuret reaction
SALT PRECIPITATION
the protein is charged positively
DYE BINDING
most commonly used;
sensitive dye for albumin
Bromcresol Green (BCG)
most specific dye
Bromcresol purple (BCP)
can be used but not specific for
albumin
Methyl Orange
more specific to albumin than methyl orange
2,4’-hydroxyazobenzene-benzoic acid (HABA
is negatively charge which is bind
to positive (albumin)
Amniotic dye
Validate if globulin is higher than albumin
ALBUMIN/GLOBULIN RATIO
globulin is higher than
albumin
seen in cirrhosis, multiple myeloma and
Waldenstroms magcroglobolinemia
Inverted A/G ratio
PROTHROMBIN TEST
prior to testing
administered vit K intramuscularly 10mg for 1-3
days.
Vitamin K Response Test
PROTHROMBIN TEST
indication that there is a problem in liver
synthetic; HEPATIC
If PROTHROMBIN time is prolonged
PROTHROMBIN TEST
prothrombin time is normal the problem
is EXTRAHEPATIC
After administration of VIT K
It differentiates intrahepatic disorder from
extrahepatic disorder
PROTHROMBIN TEST
