Tests Flashcards
NMR
bulk chemical characterization
concentration of specific elements
FTIR
functional groups within chemical structures of repeating units
GPC
polydispersity index
MW sensitive = tells size and concentration
mass spectrometry
aqueous soluble or organic soluble homopolymers
DSC
heat of crystallization/melting
thermal transition and %crystallinity
TGA
change in weight vs temperature
degradation temperature and therefore thermal processing range
XPS
surface composition
contact angle
- determines hydrophobicity/hydrophillicity of a material surface
- lower angle = more hydrophilic
+: inexpensive multiple site analysis gives uniformity info
-: no direct date on protein adsorption, needs extra analysis
ELISA
protein coating and density gives quantitative values of layering of proteins
Relative
radioimmunoassay/fluorescent tagging/light absorption
+: accurate sensitive on modified and unmodified surfaces
-: handling radiochemicals, safey issue, expensive
ellipsometry
+: accurate grafting height and protein depth
-: expensive, different results in wet or dry state
Measures total mass, doesn’t tell what is on surface
quartz crystal microbalance
info about quantity not specificity
atomic form microscopy
+: nanolevel resolution image of surface
-: tedious sample prep/setup, noncontact mode, expensive
optical microscope
light absorption and intensity gives quantitative data
SEM/TEM
very small level photon light measurement, can be at nano scale, expensive
quantity of adsorbed proteins
QCM, ellipsometry, fluorescent labeling, radiolabeling
platelent adhesion under different blood flow rate conditions
allow platelents to flow over surface ant controlled rate in an in vitro chamber for specific periods of time, use optical microscopy to image adhered platelents and analyze
measure surface factor modification
FTIR-ATR, ellipsometry, QCMB, ELISA
determine extent of albumin vs fibrinogen adsorption
flurorscence intensity, radiolabeled assay, QCMB assay
surface engineering a biomaterial surface for cell adhesion
SAMs
test to determine whether surface modification was successful
XPS, FTIR-ATR, ELISA, ellipsometry
quantify extent of modifying motifs
XPS, FTIR-ATR
ensure certain cell types for surface modification
optical or fluorescence microscopy
quantify stable adhesion
flow environment and microscopy
ensure viability of adherent cells
metabolism assay, live/dead assay
test to examine effectiveness of minimizing protein adsorption
AFM, ellipsometry, radiochemical, contact angle
in vitro experiment for protein affinity
ELISA or radiolabelling,
run separate test each time with different tagging or labeling of protein
