Tests

Question 1

NMR

Answer 1

bulk chemical characterization

concentration of specific elements

Question 2

FTIR

Answer 2

functional groups within chemical structures of repeating units

Question 3

GPC

Answer 3

polydispersity index

MW sensitive = tells size and concentration

Question 4

mass spectrometry

Answer 4

aqueous soluble or organic soluble homopolymers

Question 5

DSC

Answer 5

heat of crystallization/melting

thermal transition and %crystallinity

Question 6

TGA

Answer 6

change in weight vs temperature

degradation temperature and therefore thermal processing range

Question 7

XPS

Answer 7

surface composition

Question 8

contact angle

Answer 8

• determines hydrophobicity/hydrophillicity of a material surface
• lower angle = more hydrophilic
  +: inexpensive multiple site analysis gives uniformity info
  -: no direct date on protein adsorption, needs extra analysis

Question 9

ELISA

Answer 9

protein coating and density gives quantitative values of layering of proteins
Relative

Question 10

radioimmunoassay/fluorescent tagging/light absorption

Answer 10

+: accurate sensitive on modified and unmodified surfaces

-: handling radiochemicals, safey issue, expensive

Question 11

ellipsometry

Answer 11

+: accurate grafting height and protein depth
-: expensive, different results in wet or dry state
Measures total mass, doesn’t tell what is on surface

Question 12

quartz crystal microbalance

Answer 12

info about quantity not specificity

Question 13

atomic form microscopy

Answer 13

+: nanolevel resolution image of surface

-: tedious sample prep/setup, noncontact mode, expensive

Question 14

optical microscope

Answer 14

light absorption and intensity gives quantitative data

Question 15

SEM/TEM

Answer 15

very small level photon light measurement, can be at nano scale, expensive

Question 16

quantity of adsorbed proteins

Answer 16

QCM, ellipsometry, fluorescent labeling, radiolabeling

Question 17

platelent adhesion under different blood flow rate conditions

Answer 17

allow platelents to flow over surface ant controlled rate in an in vitro chamber for specific periods of time, use optical microscopy to image adhered platelents and analyze

Question 18

measure surface factor modification

Answer 18

FTIR-ATR, ellipsometry, QCMB, ELISA

Question 19

determine extent of albumin vs fibrinogen adsorption

Answer 19

flurorscence intensity, radiolabeled assay, QCMB assay

Question 20

surface engineering a biomaterial surface for cell adhesion

Answer 20

SAMs

Question 21

test to determine whether surface modification was successful

Answer 21

XPS, FTIR-ATR, ELISA, ellipsometry

Question 22

quantify extent of modifying motifs

Answer 22

XPS, FTIR-ATR

Question 23

ensure certain cell types for surface modification

Answer 23

optical or fluorescence microscopy

Question 24

quantify stable adhesion

Answer 24

flow environment and microscopy

Question 25

ensure viability of adherent cells

Answer 25

metabolism assay, live/dead assay

Question 26

test to examine effectiveness of minimizing protein adsorption

Answer 26

AFM, ellipsometry, radiochemical, contact angle

Question 27

in vitro experiment for protein affinity

Answer 27

ELISA or radiolabelling,

run separate test each time with different tagging or labeling of protein