Protein Interactions

Question 1

thromboembolism formation

Answer 1

blood flow over biomaterial with poor hemocompatibility = protein deposition > activation of coagulation cascade which acts on prothrombin to make thrombin, which acts on resting platelets to make activated platelets > thrombus formation > detaches, makes thromboembolism

Question 2

factors influencing protein adsorption

Answer 2

size, hydrophobicity, charge, roughness, structural stability, unfolding rate
non-covalent interactions drive protein adsorption - minimize weak interactions between surface and protein to minimize adsorption

Question 3

vroman effect

Answer 3

short times: high concentration proteins

longer times: higher affinity proteins

Question 4

quantitative surface density biophysical analyses

Answer 4

surface plasmon resonance (SPR), quartz crystal microbalance (QCMB), optical waveguid lightmode spectroscopy (OWLS)
- real time, lower sensitivity, less prep, total mass(no specificity), flow vs. static

Question 5

quantitative surface density direct labeling analyses

Answer 5

analyzes end points:
radioisotope, fluorophore
- potential labeling artifacts, high sensitivity, specificity