Biomaterial-Cell interactions

Question 1

controlling protein adsorption

Answer 1

engineer surface chemistry - non adhesive functional groups: PEG, phospholipids, carbohydrates(mannitol)

Question 2

metabolic activity measurement of cell proliferation

Answer 2

+: low sample numbers

-: non specific, residual chemical can alter cell activity

Question 3

DNA harvest measurement of cell proliferation

Answer 3

+: direct measurement of cell population

-: must work with low cell density, multiple time points/high sample volume, need standards

Question 4

cell count measurement of cell proliferation

Answer 4

+: simple

-: very inaccurate

Question 5

nucleotide incorporation measurement of cell proliferation

Answer 5

+:specific to proliferation cells, low sample number

-: time intensive, time specific

Question 6

analysis of protein expression: real time PCR

Answer 6

+: highly sensitive, highly specific, quantitative

-:standards and primers needed, cost, time intensive, gene expression does not mean protein expression

Question 7

analysis of protein expression: Western blot of expressed protein

Answer 7

+:sensitive, highly specific

-:relative values, time, difficulty in sample harvest

Question 8

analysis of protein expression: immunofluorescence imaging

Answer 8

+: simple, visual image, specific, co-localization of multiple proteins
-: not quantitative, sample bleaching

Question 9

implantation of biomaterial = ?

Answer 9

acute inflammation > chronic inflammation > granulation tissue > healing