Tests Flashcards
How to test for non reducing sugar?
Add HCL, boil and cool solution
Add sodium hydrogen carbonate
Add Benedict’s Reagent
What is observed in the Benedict's test for a reducing sugar if a reducing sugar is not present?
Solution remains blue
What is observed in the Benedict's test for a reducing sugar if a reducing sugar is present?
A red precipitate
is formed
Why is acid used in
the Benedict’s test for
a non-reducing
sugar?
It hydrolyses the
glycosidic bond
Why is sodium hydrogencarbonate used in Benedict's test for a non- reducing sugar?
Neutralises the
solution as Benedict’s
reagent doesn’t work
in acidic conditions
What is the test used
for a reducing sugar?
Add an equal amount
of benedict’s solution
Place in a water bath
for 3 minutes
What is observed in the Benedict's test for a non-reducing sugar if a non-reducing sugar is present?
Brick red precipitate
formed
How can starch be
tested for?
Adding potassium
iodide solution
What is observed in a
positive starch test?
Black/dark purple
solution is formed
Describe the method
for testing for the
presence of proteins
Add Biuret’s reagent
down the side of the
test tube
Shake
What is observed in a
positive test for proteins
Purple solution is formed