Tests Flashcards

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1
Q

How to test for non reducing sugar?

A

Add HCL, boil and cool solution
Add sodium hydrogen carbonate
Add Benedict’s Reagent

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2
Q
What is observed in
the Benedict's test for
a reducing sugar if a
reducing sugar is not
present?
A

Solution remains blue

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3
Q
What is observed in
the Benedict's test for
a reducing sugar if a
reducing sugar is
present?
A

A red precipitate

is formed

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4
Q

Why is acid used in
the Benedict’s test for
a non-reducing
sugar?

A

It hydrolyses the

glycosidic bond

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5
Q
Why is sodium
hydrogencarbonate
used in Benedict's
test for a non-
reducing sugar?
A

Neutralises the
solution as Benedict’s
reagent doesn’t work
in acidic conditions

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6
Q

What is the test used

for a reducing sugar?

A

Add an equal amount
of benedict’s solution
Place in a water bath
for 3 minutes

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7
Q
What is observed in
the Benedict's test for
a non-reducing sugar
if a non-reducing
sugar is present?
A

Brick red precipitate

formed

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8
Q

How can starch be

tested for?

A

Adding potassium

iodide solution

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9
Q

What is observed in a

positive starch test?

A

Black/dark purple

solution is formed

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10
Q

Describe the method
for testing for the
presence of proteins

A

Add Biuret’s reagent
down the side of the
test tube
Shake

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11
Q

What is observed in a

positive test for proteins

A

Purple solution is formed

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