Tests Flashcards
(11 cards)
How to test for non reducing sugar?
Add HCL, boil and cool solution
Add sodium hydrogen carbonate
Add Benedict’s Reagent
What is observed in the Benedict's test for a reducing sugar if a reducing sugar is not present?
Solution remains blue
What is observed in the Benedict's test for a reducing sugar if a reducing sugar is present?
A red precipitate
is formed
Why is acid used in
the Benedict’s test for
a non-reducing
sugar?
It hydrolyses the
glycosidic bond
Why is sodium hydrogencarbonate used in Benedict's test for a non- reducing sugar?
Neutralises the
solution as Benedict’s
reagent doesn’t work
in acidic conditions
What is the test used
for a reducing sugar?
Add an equal amount
of benedict’s solution
Place in a water bath
for 3 minutes
What is observed in the Benedict's test for a non-reducing sugar if a non-reducing sugar is present?
Brick red precipitate
formed
How can starch be
tested for?
Adding potassium
iodide solution
What is observed in a
positive starch test?
Black/dark purple
solution is formed
Describe the method
for testing for the
presence of proteins
Add Biuret’s reagent
down the side of the
test tube
Shake
What is observed in a
positive test for proteins
Purple solution is formed