testing things

Question 1

direct examination-detecting the organism is most effective in what stage of disease

Answer 1

acute disease

PCR

Question 2

ELISA detects what

Answer 2

antigens

Question 3

what are our antigen test

Answer 3

elisa
IHC
IFA

Question 4

what is the indirect examination

Answer 4

virus isolation

Question 5

what does serology do

Answer 5

detect the antibody. tells us of exposure. not current diesase

Question 6

when is serology best used

Answer 6

to see what is circulating in a population because it will tell us who out of the population has been exposed

Question 7

when does IgM peak

Answer 7

one week

Question 8

on the snap 4DX. which detect antigens and which detect antibodies

Answer 8

heartworm is antigen-true infection. all others are antibodies-exposure

Question 9

what type of test is a snap test

Answer 9

elisa

Question 10

IFA

Answer 10

detects antigen by glowing. you must see the glow

Question 11

you have a 1:2 titer and a 1:200 titer. what do they mean

Answer 11

1:2 titer has less antibody than a 1:200 because they have diluted it down to 1:200 and could still detect the antibody

Question 12

agglutination

Answer 12

detects both antibody or antigen. xlinking making clumps.

Question 13

we get a titer of 1:80. what do we do

Answer 13

one titer means nothing, run a convalescing titer to see if it stays high/increases.

Question 14

owner wants to get titers instead of vaccinating, what do you say

Answer 14

first you look for a reference range for that vaccine that is established in the literature.

Question 15

in general, what tests are more sensitive for viral antigens

Answer 15

molecular tests better than serological tests

Question 16

sensitivity. what do you trust

Answer 16

sensitive to the +, so you trust the -

Question 17

specificity. what do you trust

Answer 17

specificity gets -. so trust the +

Question 18

you take a BVD elisa test that is Positive but the BVD assay titer is negative. 1:0. what do you do

Answer 18

elisa detects antigen so the virus is there. you decide this is an acute infection because it has not had time to create antibodies

Question 19

what is the general rule if you get high antibody titers

Answer 19

do another titer 2weeks later. if it is a true infection it should increase or stay high.

Question 20

what is the risk of using serologic assays in young animals

Answer 20

maternal antibodies!

Question 21

you get a positive antibody snap test. what do you do

Answer 21

follow up titer, PCR, or look in blood smear. an antibody snap test does not mean there’s an active infection