Test 3 Review Flashcards
Describe a redox reaction. Identify the species undergoing oxidation and those undergoing reduction in such reaction.
Oxidation- species losing electrons (H)
Reduction - species gaining electrons (H)
Explain why metabolic pathways are comprised of many, small steps from reactants to products rather than just one large step.
It’s many steps so that it can be slowed and controlled reaction. If it was in one big step it would result in just a burst of unusable energy, like an explosion
Explain the roles of electron carriers in the cell and describe their general structure
NAD+/NADH(nicotinamide adenine dinucleotide): oxidized form (+charge) with H from food is reduced to produce reduced form. Or reduced form (no charge) is oxidized to produce oxidized form and H
FAD/FADH2(flavin adenine dinucleotide): oxidized from with phosphate bridge with H is reduced. Vice versa. Free energy released during transfer of H+/e- is used to drive ATP synthesis
Provide an overview of the process of aerobic respiration and where events are taking place
Aerobic respiration is the total process by which food molecules are ultimately oxidized by cells, using oxygen
C6H12O6+ 6O2——>6CO2+6H2O+ Energy(ATP), where the glucose is oxidized to CO2 and the oxygen is reduced to H2O.
Glycolysis-cytoplasm
Citric acid cycle- mitochondrial matrix
Oxidative phosphorylation- mitochondrion
Describe the glycolysis pathway
2 ATP are turned into ADP to catabolize glucose. 4 ATP and 2 NADH (+2H+) are produced, as are 2 pyruvate and 2 H2O
What is the energy investment phase in the glycolytic pathway ?
ATP is required in order to prepare and prime the phosphorylated intermediates. Then there is cleavage into 3 carbon sugars (triose): glyceraldehyde(trialdose) and DHA(ketotriose).
What is the energy pay off phase in the glycolytic pathway ?
Organic molecules are oxidized (G3P), then ATP is produced and pyruvate is formed
Describe the fate of pyruvate in both aerobic and anaerobic environments
Aerobic: pyruvate turns into Acetyl CoA(mitochondria) and enters the citric acid cycle
Anaerobic: either ethanol or lactate fermentation occurs (cytosol)
What is an obligate aerobe?
An organism/cell that requires oxygen
What is an obligate anaerobe?
An organism/cell that can’t use oxygen and it’s poisonous to them
What is a facultative anaerobe?
An organism/cell that can survive with or without oxygen
Describe in detail the structure of the mitochondria. Explain how this structure is related to its functions
-outer and highly folded inner membrane (cristae)
- inter membrane space and matrix
-found in almost all eukaryotes
-# varies p/cell (500+ in liver,50 in less active)
- localized to regions of high metabolic activity (contraction in muscle cells)
Provide an overview of aerobic respiration. Describe specifically where all the events are taking place
-fuel molecules (organic) are oxidized to inorganic molecules to produce usable energy
- C6H12O6——->6O2+6H2O + Energy (ATP)
- Free energy released during electron transfer used to drive ATP synthesis
- Glycolysis : cytoplasm
- Citric Acid Cycle : mitochondrial matrix
- Oxidative Phosphorylation: inner mitochondrial membrane
Describe PDH and the oxidative decarboxylation of pyruvate by PDH
-before entering TCA, pyruvate is oxidized by pyruvate dehydrogenase
-large multimeric quaternary protein complex several enzymes(3), coenzymes and regulatory proteins
1) catalyzes oxidative decarboxylation of pyruvate (removes CO2)
2) pyruvate is oxidized ; NAD+ is reduced to NADH
3) remaining carbon (acetate) attached to coenzyme A—> acetyl CoA (thioester bond)
Describe the form and fate of the carbons in the Kreb cycle
Acetyl CoA (2C) + oxaloacetate ( 4C; 1st acceptor) —-> Citrate (6C) (1st intermediate)
-2CO2s released p/cycle
- electron carriers are reduced
-1 ATP p/ cycle via substrate level phosphorylation
- inputs : 2 Acetyl p/ glucose
- net result ; 3 NADH, 1 ATP, 1 FADH, releases 2CO2
- turns twice for each molecule of glucose ( 4 CO2 released p/ glucose other 2 in PDH
Explain the role of Coezyme A in aerobic respiration. Does it take place in the Kreb’s cycle ?
-combines with acetate to form Acetyl CoA
- no it takes place before Kreb’s cycle
Explain the driving force behind electron movement through the electron transport chain. What drives the concomitant movement of protons across the IMM?
Electrons are transferred energetically downhill. Free E released by the movement of electrons through the ETC is coupled to the transport of portions into the inter membrane space
Explain how membrane structure is related to membrane function in chemiosmosis
The phospholipid bilayer. If the electron carriers (proteins ) weren’t there, it wouldn’t happen
What is substrate level phosphorylation ?
Formation of ATP via direct transfer of phosphate group from a phosphorylated substrate to ADP
Mechanism is very different from oxidative phosphorylation
Happens during glycolysis and Kreb’s cycle
What is oxidative phosphorylation ?
Formation of ATP via oxygen dependent electron transport
Process is driven by an electrochemical gradient formed using free E released during electron transport
Summarize the net ATP yield from the oxidation of glucose ion aerobic respiration. Compare that to the net ATP yield from glycolysis alone
Glycolysis : 2 ATP
Aerobic Respiration: 36-38 ATP
-2 from glycolysis
-2 from Kreb’s cycle
- 32-34 from oxidative phosphorylation
Describe the pathways of alcoholic fermentation and lactate( lactic acid) fermentation. Explain why fermentation is necessary
Formation still produces ATP even if oxygen isn’t present. It also allows the electron carriers like NADH to drool off their H someplace, so they can do their job again
Explain why fermentation is important economically
It helps make cheese yogurt, beer, wine, nail polish, vinegar…ect
Compare the cellular processes of fermentation and aerobic respiration. Speculate on the types of cells/organism that carry out these processes
Cellular respiration: eukaryotes
Fermentation: Bacteria and smaller organisms (yeast)
The big picture of chemotrophs energy metabolism by linking together the processes of glycolysis, kreb’s cycle, ETC and oxidative phosphorylation
Basically if we break down 1 molecule of glucose for energy, it’s turned to 2 pyruvate and the Kreb’s cycle runs twice, netting 36-38 atp aerobically. Fermentation occurre in the cytosol. Glycolysis occurs, then NADH reduces the pyruvate to make lactate. For alcohols, the carboxyl group is cleaved off pyruvate, releasing CO2, then it’s reduced to make ethanol
In glycolysis, what happens to glucose in order to produce two pyruvate molecules ?
A) anabolic process
B) catabolic process
B) catabolic process
In glycolysis, glucose is …. And the overall process is ……..
A) reduced, exergonic
B) reduced, endergonic
C) oxidized, exergonic
D)oxidized,endergonic
C) oxidized, exergonic
NAD+ is the …. Form of NADH
A) oxidized
B) reduced
A) oxidized
During glycolysis, electrons/ protons are ….. glyceraldehyde-3-phosphate (G3P) by NAD+, thereby….. G3P
A) donated to , oxidizing
B) removed from , oxidizing
C) donated to, reducing
D) removed from , reducing
B) removed from , oxidizing
The conversion of pyruvate to Acetyl CoA involves the enzyme complex pyruvate dehydrogenase (PDH). Based on the name of this enzyme complex, what happens to pyruvate ?
A) it is reduced
B)it is oxidized
C) water is removed
D) water is added
E)none of the above
B)it is oxidized
PDH is a large multimeric complex. What is the highest level of protein structure in PDH?
A) primary
B) secondary
C) tertiary
D) quaternary
E) not enough information given
D) quaternary
Citrate, the first intermediate of the citric acid cycle, will have …. Carbons
A) 2
B) 4
C) 5
D) 6
E) 8
D) 6
The acceptor molecule, oxaloacetate, is regenerated in one full turn of the citric acid cycle. How many CO2 molecules are therefore released during each turn of the cycle ?
A) 1
B) 2
C) 3
D) 4
E) 5
B)2
How many times will the TCA cycle turn per each glucose molecule ?
A) 1
B) 2
C) 3
D) 4
E) 6
B) 2
Per glucose, how many molecules of CO2 are released from the TCA cycle ?
A) 6
B) 4
C) 3
D) 2
E) 1
B) 4
Electrons will spontaneously flow from complexes with …. electronegativity (EN) to complexes with ….. electronegativity (EN)?
A) greater; lower
B) lower; greater
C) neither of the above. EN differences don’t influence movement of electrons
B) lower; greater
When electrons are transferred to a more electronegative molecule, what happens ?
A) the more electronegative molecule is reduced, and energy is consumed
B) the more electronegative molecule is oxidized, and energy is consumed
C) the more electronegative molecule is reduced , and energy is released
D) the more electronegative molecule is oxidized, and energy is released
E) the more electronegative molecule is reduced and entropy decreases
C) the more electronegative molecule is reduced , and energy is released
How does a low pH correspond to [H+]
A) low pH= low [H+]
B) low pH= high [H+]
C) pH doesn’t indicate anything about [H+]
B) low pH= high [H+]
Free E released by the movement of electrons through the ETC is coupled to the transport of protons into the inter membrane space. This means the pH of the matrix is …… then the pH of the inter membrane space
A) higher
B) lower
C) the same
A) higher
If the spontaneous movement of electrons is coupled to the transport of protons across the inner mitochondrial membrane (IMM), what mechanism is being used to transport the protons?
A) simple diffusion
B) facilitated diffusion
C) osmosis
D) active transport
E) exocytosis
D) active transport
Protons will move from the inter membrane space into the matrix by which type of transport mechanism ?
A) diffusion
B) facilitated diffusion
C) active transport
D) osmosis
E) exocytosis
B) facilitated diffusion
During fermentation , pyruvate is …….. by NADH
A) oxidized
B) reduced
C) neither oxidized nor reduced
B) reduced