Test 2 Review

Question 1

What is first complex in electron transport of oxidative phosphorylation in respiring E. coli?

Answer 1

NADH dehydrogenase

Question 2

What is the rxn of NADH dehydrogenase?

Answer 2

(1st e transport complex)

NADH -> 2e + NAD+ + H+

Question 3

What is the 3rd complex in e transport of oxidative phosphorylation in respiring E. coli?

Answer 3

Cytochrome b-c1 complex

Question 4

What is the 4th complex in e transport of oxidative phosphorylation in respiring E. coli?

Answer 4

Cytochrome oxidase complex

Question 5

What is the rxn of cytochrome oxidase complex?

Answer 5

1/2 O2 + 2H+ + 2e -> H2O

Question 6

By what method is energy harvested by e transport, converted to ATP?

Answer 6

F0F1 ATP synthase

Question 7

Describe F0F1 ATP synthase mechanism

Answer 7

E transport is used to pump protons across the plasma membrane of respiring E coli into the periplasm, creating a proton gradient. The energy of the proton gradient is then “harvested” to synthesize ATP. Protons flow down gradient into the cytoplasm thru the F0F1 ATP synthase. As they do so, they turn the rotor component of the F0 complex, which is connected to the “stalk” or “shaft” of the ATP synthase. As the stalk turns inside the catalytic head, it drives conformational changes in the α/β catalytic heads that drive ATP synthesis

Question 8

In what state (reduced or oxidized), in the absence of O2, all components of the mitochondrial E transport chain will accumulate?

Answer 8

reduced

Question 9

When O2 is re-added to mitochondria after a period of anoxia, cytochrome C will become more [reduced or oxidized] [ before or after] those in NADH dehydrogenase?

Answer 9

oxidized and before

Question 10

In which cellular membrane would you find protein complexes of E Transport chain of photosynthesis in a plant cell?

Answer 10

Chloroplast thylakoid membrane

Question 11

What cellular compartment uses 2 protons from product of H2O synthesis?

Answer 11

Chloroplast Stroma

Question 12

What is the chemical rxn for Chloroplast Stroma?

Answer 12

H2O -> 1/2 O2 + 2H+

Question 13

After Chloroplast Stroma rxn and addition of 2 protons what protein complex comes next?

Answer 13

Photosystem II

Question 14

What enzyme is used for synthesis of H2O in Chloroplast Stroma?

Answer 14

water-splitting enzyme

Question 15

What protein/complex comes after Photosystem II?

Answer 15

Photosystem I

Question 16

What protein/complex utilize 2 protons?

Answer 16

Chloroplast Stroma and Photosystem I