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Microbiology > Test 2 > Flashcards

Test 2 Flashcards

1
Q

An anti microbial that inhibits cell wall synthesis will result in

A

Cells become more susceptible to osmetic pressure.

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2
Q

The bacterial chromosome is

A

Both circular and found in nucleoid

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3
Q

Which of the following statements is true of bacterial plasmids?

A

They are small circular DNA molecules that can replicate autonomously

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4
Q

Beta-Lactam antibiotics have an effect on which of the following types of cells

A

Bacterial Cells

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5
Q

Which of the following is NOT a target of drugs that inhibit protein synthesis

A

interference with alanine-alanine bridges

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6
Q

Which of the following must be removed from eukaryotic mRNA molecule before it can be translated?

A

Intron

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7
Q

What is involved in translation

A

mRNA

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8
Q

Semi conservative DNA replication means that

A

each daughter dna molecule is composed of one original strand and one new strand

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9
Q

Which of the following statements regarding eukaryotic transcription is FALSE

A

A guanine is added to the 3 end and a poly-A tail is is added to the 5 end of the mRNA

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10
Q

A charged tRNA first enters the ribosomal ______ site and then moves into the _______ site

A

A, P

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11
Q

DNA helicases

A

proofread DNA molecules

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12
Q

Which of the following statements about the zone inhibition is FALSE?

A

the larger the zone, the more resistant the organism is.

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13
Q

Probiotics

A

Are an alternative to the use of chemotherapy involving microbial antagonism

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14
Q

Most drugs that inhibit the synthesis of the cell wall act by

A

Preventing the cross linkage of NAM subunits

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15
Q

Most broad spectrum antibiotics act by

A

Inhibiting protein synthesis

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16
Q

It is inappropriate to prescribe antibacterial agents to treat colds or flu because

A

These diseases are caused by viruses

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17
Q

Which of the following statements is true of selective toxicity

A

Selective toxicity takes advantage of structural and/or metabolic differences between host and pathogen

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18
Q

Which of the following is NOT a characteristic of Okazaki fragments?

A

The are longer in eukaryotic cells

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19
Q

Which of the following are considered to be frameshift mutations?

A

Deletions and insertions

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20
Q

The horizontal transfer process known as transduction

A

involves a virus

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21
Q

If the codon AAA is changed to AAG, it still codes for the animo acid lysine, this is an example of a

A

Silent Mutation

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22
Q

Put the following steps in the correct order needed to produce a recombinant vector containing a human gene inert

A

2, 3, 1, 4

23
Q

Which of the following restriction enzyme sites would produce blunt-ended fragments

24
Q

Which of the following is essential in PCR

A

both DNA primers and DNA polymerase

25
If researcher used Escherichia coli DNA polymerase instead of the Thermus Aquaticus DNA polymerase in the PCR procedure, what would be the result
PCR would stop after one cycle
26
Which of the following would be an appropriate sequence of temperatures for PCR
94c, 65c, 72c,
27
If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?
64
28
Which of the following devices is used for PCR?
a thermocycler
29
Which of the following procedures might be used to detect the presence of genetic sequences of a virus in a patience blood?
xenotransplantation
30
An effective tool for screwing large numbers of genetic sequences at once is known as
Microarray
31
Which of the following statements is true of disinfectants?
Disinfectants are used on inanimate surfaces.
32
Which of the following is an example of sanitazation
A public toilet is treated with disinfectants
33
In gel electrophoresis, DNA molecules moves toward the ____ electrode because they have an overall ____ charge
Positive, Negative
34
Which of the following would NOT be bacteriostatic?
Autoclaving
35
Which of the following is the most appropriate pairing of microbe and biosafety level?
methicillin-resistant Staphylococcus aureus (MRSA), BSL 2
36
Which of the following types of radiation is nonionizing and has the shortest wavelength?
ultraviolet light
37
Which of the following describes flash pasteurization?
heating at 72c for 15 seconds
38
Which of the following procedures is currently the standard test used in the U.S for evaluationg the efficiency and disinfectants?
use-dilution test
39
Which of the following would be used to sterilize a mattress?
Ethylyne Oxide
40
A librarey cloned sequences representing the expressed genes of an organism is known as
cDNA library
41
Inhibition of cell wall synthesis
Cephalosporins
42
Disruption of cytoplasmic membranes
Polymyxin
43
Inhibition of nucleic acid synthesis
Actinomycin
44
Inhibition of metabolic pathways
Sulfonamides
45
Inhibition of protein synthesis
Aminoglycosides
46
The abbreviation _____ stands for the smallest amount of a drug that will inhibit the growth and reproduction of a pathogen
MIC
47
Extrachromosomal pieces of DNA called _____ promote horizontal transfer of genes among bacteria and contribute to the spread of antimicrobial resistance
R-Plasmid
48
Nucleic acid molecules used to deliver new genes to cells are called ______
Vectors
49
A(n) _______ is a specific sequence of nucleotides that codes for a protein or molecule.
Gene
50
The _____ of a transfer RNA molecule is complementary to a codon in a RNA molecule
Anti-Codon
51
_______. first isolated from bacterial cells, cut DNA molecules at specific sites.
Restriction Enzymes
52
________ means that a given antimicrobial agent is more toxic to a pathogen than to the host being treated
Selective Toxicity
53
Sterilization procedures generally focus on inactivating or eliminating bacterial _______.
Endospores

Microbiology (5 decks)

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