LAB Flashcards

1
Q

_______ is the lowest temperature that kills all cells in broth in 10 mins

A

Thermal Death Point

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2
Q

To kill the microbes which may be present in the dairy cow ________ method is commonly used in milk industry.

A

Pasteurization

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3
Q

Autoclaving is a process that uses ______ heat methods

A

Moist

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4
Q

Give one possible reason that why just counting the number of bacteria is not enough for judging the safety of the product.

A

Low number of bacteria does not mean Non-Pathogenic

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5
Q

Calculate the concentration of bacteria in milk sample.

A

50x10^4

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6
Q

Identify the types shown in the blood smear

A

RBC, Platelets, Lymphocytes, Neutrophils and Basophils

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7
Q

_______ reduce the number of microbes on living surfaces, such as our skin, while _____ reduce the number of microbes on non-living surfaces such as surface of lab benches.

A

Antiseptics, Disinfectants

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8
Q

Triple sugar iron (TSI) agar is used to diffrentiate and identify gram _____ enteric microbes.

A

Negative

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9
Q

The clear zone shown here around the antibiotic disc is called _______.

A

Zone of Inhibition

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10
Q

An inverted inner vial is placed inside the culture tube in order to detect gas production is called ______ tube.

A

Durham Tube

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11
Q

Compare with other agents tested in the following experiment, _______ is most effective in inhibiting the growth of Stahylococcus aureus because it shoes the largest________.

A

Hydrogen Peroxide, Zone of inhibition

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12
Q

______ are viruses that infect bacteria. Identification of bacterial strains using inefecctivity by different _____ is called ______.

A

Bacteriaphages, phages, phage typing

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13
Q

Identify the cell type ______. This type of cell is usually elevated during parasitic infection and allergic reactions.

A

Eosinophil

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14
Q

2 green 1 Blue

A

Enterobacter, Permeases and Alkaline

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15
Q

List 3 anti-biotics used in the lab and describe their mechanism of action.

A

Penicilin - disrupts cell synthesis
Tetracyclines - protein synthesis
Streptomycin

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16
Q

How bacteria become resistant to antibiotics?

A

New mutations of chromosomal genes and acquisition of R-Plasmids via transformation

17
Q

Which of the following bacteria are infected by T-phage?

A

E. coli B.

18
Q

______ is the standard method used for antibiotic-sensitivity testing.

A

Kirby-Bauer method