Tertiary structure Flashcards
what are tertiary structures ultimately determined by?
amino acid sequence
environment in which protein is found
what kind of environment do soluble proteins require
aqueous environments
where do membrane protein structures fold properly, and how can this environment be imitated?
membrane
detergents
which forces are critical in determining tertiary structure
noncovalent
hydrophobic
van der waals
H-bonds
ionic interactions
what is the native conformation
low-energy functional state
what is the delta G for folding into native conformation
-100 kJ/mol
T or F: there is only one stable functional state of native conformation
False
how are functional states of native conformation created?
ligands, flexible portions of proteins, breathing
which technique can yield general characteristics of secondary structure
circular dichroism
which technique can yield detailed atomic information
x-ray crystallography, protein NMR
how does circular dichroism work?
differential absorption of left and right circularly polarized light
what type of structure is most sensitive to circular dichroism
secondary
what can be seen through CD spectra
alpha helices, beta sheets, random coils and irregular structures
what kind of experiments is CD particularly useful for
denaturation/ renaturation
how does xray crystallography work
protein preparations (pure) require pure samples which are then exposed to X-rays
