quaternary structure

Question 1

how is hemoglobin described

Answer 1

tetramer or dimer of alpha beta protomers

Question 2

how are the subunits of hemoglobin arranged

Answer 2

symmetric pairs

Question 3

what is the individual units that form multimers

Answer 3

subunits

Question 4

what is the individual units that form oligomers

Answer 4

protomers

Question 5

what are the two types of symmetry in protomers

Answer 5

rotational and translational

Question 6

what are the 3 characteristics of guanidinium and urea

Answer 6

chaotropic
water solube
disrupt hydrophobic interactions

Question 7

what is consistent for both the reducing agents of interest

Answer 7

thiol group

Question 8

what are the two reducing agents of interest

Answer 8

BME and DTT

Question 9

what do reducing agents do (2)?

Answer 9

reduce disulfide bonds and become oxidized as part of the reaction

Question 10

how might a protein become locked

Answer 10

disulfide bonds reform before removal of denaturant

Question 11

what kind of process is protein folding

Answer 11

cooperative

Question 12

when does protein assume the molten globule state

Answer 12

after formation of secondary but before tertiary

Question 13

which has more entropy, unfolded or folded

Answer 13

unfolded

Question 14

which has more energy, folded or unfolded

Answer 14

foldede

Question 15

what may assist in protein folding

Answer 15

molecular chaperones and isomerases