shit i actually will not remember lol Flashcards
which AA is found at polar and non polar boundary
tryptophan
what is the functional group name in methionie
thiol ether
what does the average mass of aa value take into account
mass and frequency of incorporation
list the aromatic amino acids in order of decreasing polarity
histidine, tyrosine, tryptophan, phenylalanine
what wavelength do tyr and trp absorb wavelengths at
280 nm
what is the 3 and 1 letter code for selenocysteine
sec u
what is the 3 and 1 letter code for pyrrolysine
pyl o
how do biological systems ensure pH changes do not exceed the optimal range
buffer
what does the henderson equation describe
shape of the titration curve for all weak acids
what does the henderson equation relate
pH pka buffer concentration
what is the name for proteins with prosthetic groups
conjugated proteins
what information is there in a sequence
structural info (not 100%) but secondary, transmembrane helices, signal peptide sequences
cell localization
what do sequence alignments provide
function, structure and evolutionary info
what do gaps represent at the genetic level
insertions or deletions
what do gaps represent at the structural level
loops
degree of similarity in sequence alignments represents…..
length of time since divergence
orthologs arise out of ….
speciation events
paralogs arise out of…..
gene duplication events
what are compounds separated by in differential centrifugation
sedimentation rate
which complexes sediments first in differential centrifugation
large ones
what kind of process is density centrifutation
equillibirum
how is the eluent monitored in chromatography
light absortbance 280 nm
what does the migration rate in electrophoresis depend on
charge/mass ratio
which chemical breaks disulfide bonds
DTT
where is the net dipole pointing in a peptide bond
towards the oxygen
what is the shape of the peptide bond?
planar
what is the bond angle in an extended chAIN
180
describe the H-bonding patterns in stable forms of secondary structures?
repeating
describe the difference in antiparallel vs parallel beta sheets
repeat period is shorter for paraellel
H-bonding pattern is different
are beta sheets planar?
nah
what are beta turns characterized by
backbone H-bonds
what is a use for beta turns
connect antiparallel beta strands
what ultimately determines tertiary structure
sequence & environment
which forces are most critical in determining tertiary structure
non-covalent
what does x-ray crystallography provide
info about electron density, used
what does x-ray crystallography provide
info about electron density, used TO BUILD MODELS
what is the crystal used to generate in x-ray crystallography?
what do these do
diffraction pattern
what is the crystal used to generate in x-ray crystallography?
what do these do
diffraction pattern3
3d electron density map revealing location of atomic nuclei
what are the pros of xray crystallography
detailed
rapid
large
what are the cons of xray crystallography
crystal growth
diffraction
noH
static
what does magnetic coupling provide information about
distance between atoms
what are the pros of protein NMR
dynamic
in solution
what are the cons of protein NMR
difficult for large
synthesis of peptides containing isotopes can be expensive
