Term 1- Lec 1&2- AA, pH, and Proteins

Question 1

Which AA’s are Non-Polar

Answer 1

Glycine, Alanine, Leucine, Isoleucine, Valine, Methionine, Phenylalanine, Proline, Tryptophan

Question 2

Which AA’s are Uncharged Polar

Answer 2

Serine, Threonine, Tyrosine, Glutamine, Asparagine

Question 3

Which AA’s are Acidic Charged Polar

Answer 3

Glutamic Acid (Glutamate), Aspartic Acid (Aspartate)

Question 4

Which AA’s are Basic Charged Polar

Answer 4

Histidine, Arginine, Lysine

Question 5

Functional Group and Interactions of Glycine

Answer 5

Hydrogen (achiral). Hydrophobic interactions

Question 6

Functional Group and Interactions of Alanine

Answer 6

Methyl. Hydrophobic interactions

Question 7

Functional Group and Interactions of Leucine

Answer 7

Branched chain hydrocarbon. Hydrophobic interactions

Question 8

Functional Group and Interactions of Isoleucine

Answer 8

Branched chain hydrocarbon. Hydrophobic interactions

Question 9

Functional Group and Interactions of Valine

Answer 9

Branched chain hydrocarbon. Hydrophobic interactions

Question 10

Functional Group and Interactions of Methionine

Answer 10

Contains S. Hydrophobic interactions

Question 11

Functional Group and Interactions of Phenylalanine

Answer 11

Contains an Aromatic Ring. Hydrophobic interactions

Question 12

Functional Group and Interactions of Tryptophan

Answer 12

Contains an Aromatic Ring. Hydrophobic interactions

Question 13

Functional Group and Interactions of Proline

Answer 13

Is an Imino Acid. Hydrophobic interactions

Question 14

What is the difference Methionine and Tryptophan from the rest of the Non-Polar AA’s?

Answer 14

They both contain a polar atom, but the polarity is masked by the surrounding hydrocarbons

Question 15

Functional Group and Interactions of Serine

Answer 15

Hydroxyl. Hydrogen Bonding

Question 16

Functional Group and Interactions of Threonine

Answer 16

Hydroxyl. Hydrogen Bonding

Question 17

Functional Group and Interactions of Tyrosine

Answer 17

Hydroxyl. Hydrogen Bonding

Question 18

Functional Group and Interactions of Glutamine

Answer 18

Carboxamide (-CONH2). Hydrogen Bonding

Question 19

Functional Group and Interactions of Asparagine

Answer 19

Carboxamide (-CONH2). Hydrogen Bonding

Question 20

Functional Group and Interactions of Cysteine

Answer 20

Sulfhydryl. Hydrogen Bonding and Disulfide Bonding

Question 21

Functional Group and Interactions of Glutamic Acid (Glutamate)

Answer 21

Carboxyl Group. Ionic Bonding and Hydrogen Bonding

Question 22

Functional Group and Interactions of Aspartic Acid (Aspartate)

Answer 22

Carboxyl Group. Ionic Bonding and Hydrogen Bonding

Question 23

Functional Group and Interactions of Histidine

Answer 23

Basic Groups. Ionic Bonding and Hydrogen Bonding

Question 24

Functional Group and Interactions of Arginine

Answer 24

Basic Groups. Ionic Bonding and Hydrogen Bonding

Question 25

Functional Group and Interactions of Lysine

Answer 25

Basic Groups. Ionic Bonding and Hydrogen Bonding

Question 26

Equation for pH

Answer 26

pH = -log( [H+] )

Question 27

Equation for Ka

Answer 27

Ka = ( [H+] • [A-] ) / [HA]

Question 28

Equation for pKa

Answer 28

pKa = -log( Ka )

Question 29

Henderson-Hasselbach Equation

Answer 29

pH = pKa + log( [A-] / [HA] )

Question 30

pH = pKa

Answer 30

50/50 [A-] & [HA]

Question 31

pH = pKa - 1

Answer 31

91% HA

Question 32

pH = pKa + 1

Answer 32

91% A-

Question 33

1º protein structure

Answer 33

AA sequence

Question 34

2º protein structure

Answer 34

∂-helices, ß-sheets, and Loops

Question 35

3º protein structure

Answer 35

Protein folding. Hydrophobic interactions and Covalent/Non-covalent bonding

Question 36

4º protein structure

Answer 36

Multiple polypeptide chains binding together to form the subunits of one active protein

Question 37

Super 2º structure- name and description

Answer 37

Motifs. Recognizable folding patterns involving 2+ 2º structures. i.e. ß-barrel

Question 38

Why are super 2º structures not 3º structures?

Answer 38

Motifs do not hold their structures by themselves. They require 3º structure to remain motifs.