Temporary Mounts Flashcards
1
Q
Dissolves non-fungal material, digest debris, to make fungal elements
more visible (acts as a clearing agent)
A
10-20% KOH Concentration
2
Q
Skin, hair
A
10% KOH
3
Q
Nails (hard tissues)
A
20% KOH
4
Q
Most widely used staining method for fungi
Acts as both preservative and stain
A
Lactophenol Cotton Blue
5
Q
Lactic Acid
A
Preservative
6
Q
Phenol
A
Killing agent
7
Q
Cotton Blue
A
Stain
8
Q
Negative stain renders the organism colorless and colors the background
A
India Ink / Nigrosin
9
Q
India Ink / Nigrosin demonstrates capsule of
A
Cryptococcus neoformans
10
Q
Fluorochrome dye – best for direct microscopy
A
Calcofluor White Stain
