Permanent Mounts Flashcards
All fungi are gram positive
Gram Stain
Crystal Violet + Ammonium Oxalate = _____
Hucker Modification
to demonstrate details of fungal elements
It is useful in staining tissue specimens
Detects fungi in tissues
Periodic Acid Schiff
Periodic Acid Schiff fungi color
pink-red color bright
magenta
Periodic Acid Schiff background color
green
detects fungi in histologic sections; brown to black.
Gomori Methenamine Silver
Gomori Methenamine Silver outline color
black
Gomori Methenamine Silver internal parts color
pink black
Gomori Methenamine Silver background color
light green
Gomori Methenamine Silver detects
Candida
Aspergillus
Demonstrates melanin
Gomori Methenamine Silver
detection of Nocardia and Blastomyces dermatitidis
Acid Fast (Kinyoun’s)
agent of systemic mycoses
Blastomyces dermatitidis
is a branching bacteria, not fungi
Nocardia
for blood parasites
Giemsa
for bloodstain; used in hematology
Wright’s
Giemsa or Wright’s stain demonstrates
Histoplasma Capsulatum
Demonstrates Malassezia furfur
- causative agent of Tinea versicolor, Pityriasis versicolor (an-an)
Acridine Orange
Detects hyphal pigmentation – to determine if hyaline or dematiaceous
Fontana Masson
H&E
Acridine Orange fungal element color
green fluorescent
Acridine Orange epithelial cell color
orange
non pigmented/lightly
pigmented
hyaline
darkly pigmented
Dematiaceous
Demonstrates Cryptococcus neoformans
Mayer’s Mucicarmine
Gridley’s Stain Hyphae and yeasts color
dark blue-pink
Gridley’s Stain Tissues
deep blue
Gridley’s Stain background
yellow
To detect fluorescence
Wood’s Lamp (UVL)
