Diagnostic Mycology

Question 1

In case of delay specimens all specimens may be refrigerated for a short
period of time, except

Answer 1

skin specimen
blood
CSF

Question 2

collected by aspiration; use of cotton swabs may give false positive
microscopic results.

Answer 2

pus

Question 3

may be collected using a fine needle and inoculated at bedside.

Answer 3

Corneal scrapings

Question 4

Sputum, bronchial washings, bronchial lavage

Answer 4

Respiratory Tract Secretions

Question 5

Respiratory Tract Secretions: for recovery of fungal agents causing systemic mycoses like

Answer 5

Histoplasma capsulatum

Question 6

For detection of Cryptococcus neoformans in
suspected fungal meningitis
(immunocompromised patients)

Answer 6

csf

Question 7

for smear and cultures

Answer 7

centrifugation

Question 8

used for serology

Answer 8

supernatant

Question 9

for micro; if tubes are insufficient, prioritize micro

Answer 9

tube 2

Question 10

uses pore diameter 0.45micra

Answer 10

filtration

Question 11

Use of BACTEC; to rule out disseminated
infections

Answer 11

blood

Question 12

Vaginal secretions should be screened for yeast
and incubated at

Answer 12

30 deg C for 7 days

Question 13

Infected hairs

Answer 13

plucked

Question 14

Skin surface

Answer 14

scraped

Question 15

Discolored or hyperkeratotic areas of nail

Answer 15

nail clipping

Question 16

Must be minced or use high speed blender

To retrieve cytoplasmic content of the tissue

Tissue samples must be inoculated on agar not
on broth

Answer 16

Tissue
Bone
Marrow
Sterile Body Fluids